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Gene Expression Data
Assay Details
Assay
Reference: J:130330 Ralston A, et al., Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo. Dev Biol. 2008 Jan 15;313(2):614-29
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3777249
Gene symbol: Eomes
Gene name: eomesodermin
Antibody: anti-Tbr2 (AB2283)
Detection system: Secondary antibody
Results
Specimen 6A: embryonic day 3.0 (more )
Note: 16-cell embryo. Double labeled: red - Eomes; white - Cdx2 (assay MGI:3777138).
Structure Level Pattern Image Note
TS4: compacted morula Absent 6A Eomes Expression is not present in the nuclei.
Specimen 6B: embryonic day 3.5 (more )
Note: This is a blastocyst stage embryo. Double labeled: red - Eomes; white - Cdx2 (assay MGI:3777138).
Structure Level Pattern Image Note
TS4: trophectoderm Present Non-Uniform 6B Eomes Expression is present in the nuclei.
TS4: inner cell mass Absent 6B Eomes
Specimen 6C: embryonic day 4.5 (more )
Note: This is a blastocyst stage embryo.
Structure Level Pattern Image Note
TS5: polar trophectoderm Present Non-Uniform 6C Expression is present in the nuclei.
TS5: mural trophectoderm Present 6C Expression is downregulated in the mural trophectoderm of a minority of embryos.
TS5: inner cell mass Absent 6C
Specimen 6D: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: extraembryonic ectoderm Present 6D Expression is present in nuclei.
TS9: extraembryonic visceral endoderm Present 6D Expression is present in nuclei.
TS9: primitive streak Present 6D Expression is present in nuclei.
Specimen 6E: embryonic day 3.5; Cdx2tm1Fbe/Cdx2tm1Fbe (more )
Note: This is a blastocyst stage embryo.
Structure Level Pattern Image Note
TS4: trophectoderm Absent 6E Eomes Expression is not detected in trophectoderm nuclei.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory