About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:130330 Ralston A, et al., Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo. Dev Biol. 2008 Jan 15;313(2):614-29
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3777138
Gene symbol: Cdx2
Gene name: caudal type homeobox 2
Antibody: anti-Cdx2 (CDX2-88)
Detection system: Secondary antibody
Results
Specimen 1A: embryonic day 1.0 (more )
Note: 2-cell embryo.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Absent 1A No expresssion was detected in any of the 20 embryos examined.
Specimen 1B: embryonic day 2.0 (more )
Note: 4-cell embryo.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent 1B No expresssion was detected in any of the 7 embryos examined.
Specimen 1C: embryonic day 2.5 (more )
Note: 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted 1C Expression is present in two of the three nuclei visible in this section. In some embryos at this stage expression was present in all the nuclei.
Specimen Not shown: embryonic day 2.5 (more )
Note: 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent Of the 30 embryos examined at this stage, 17 lacked expression. In most cases (13 of 17) these embryos had yet to compact.
Specimen 1D: embryonic day 3.0 (more )
Note: 16-cell embryo.
Structure Level Pattern Image Note
TS4: compacted morula Present Regionally restricted 1D Expression is present in some, but not all, nuclei of this embryo. For many embryos at this stage, though, expression was present in all the nuclei.
Specimen 1E: embryonic day 3.5 (more )
Note: 32-cell embryo.
Structure Level Pattern Image Note
TS4: blastocyst Present 1E Expression is present in all nuclei of this embryo; its level varies. In some embryos at this stage, not all nuclei showed expression. In those cases the nuclei with undetectable expression levels were located inside the embryo.
Specimen 1F: embryonic day 3.5 (more )
Note: 30-cell embryo.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 1F Expression is present in some, but not all, nuclei.
TS4: trophectoderm Present 1F
Specimen 2A: embryonic day 2.5 (more )
Note: 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Weak Regionally restricted 2A Expression is present in the nuclei.
Specimen 2B: embryonic day 3.0 (more )
Note: 16-cell embryo.
Structure Level Pattern Image Note
TS4: compacted morula Present Non-Uniform 2B Expression is present within the nuclei; its level varies. Outside nuclei tend to have higher expression levels than inside nuclei.
Specimen 2C: embryonic day 3.5 (more )
Note: 32-cell embryo.
Structure Level Pattern Image Note
TS4: blastocyst Present Non-Uniform 2C Expression is present within the nuclei; its level varies. Outside nuclei tend to have higher expression levels than inside nuclei.
Specimen 4F: embryonic day 3.0 (more )
Note: 14-cell embryo. Double labeled: red - Cdx2; green - Krt8 (assay MGI:3777238).
Structure Level Pattern Image Note
TS4: compacted morula Present Non-Uniform 4F Cdx2 Expression is present in some, but not all, nuclei.
Specimen 4G: embryonic day 3.5 (more )
Note: 33-cell embryo. Double labeled: red - Cdx2; green - Krt8 (assay MGI:3777238).
Structure Level Pattern Image Note
TS4: blastocyst Present Non-Uniform 4G Cdx2 Some inside cells have reduced expression levels.
Specimen 5A: embryonic day 3.5 (more )
Note: This is a blastocyst stage embryo. Double labeled: white - Cdx2; red - Krt8 (assay MGI:3777238).
Structure Level Pattern Image Note
TS4: trophectoderm Present 5A Cdx2
Specimen 5B: embryonic day 3.5; Cdx2tm1Fbe/Cdx2tm1Fbe (more )
Note: This is a blastocyst stage embryo. Double labeled: white - Cdx2; red - Krt8 (assay MGI:3777238).
Structure Level Pattern Image Note
TS4: inner cell mass Absent 5B Cdx2
TS4: trophectoderm Absent 5B Cdx2
Specimen 5C: embryonic day 3.0 (more )
Note: 16-cell embryo. Double labeled: white - Cdx2; red - Krt8 (assay MGI:3777238).
Structure Level Pattern Image Note
TS4: compacted morula Present 5C Cdx2 Expression is present in outside cells.
Specimen 5D: embryonic day 3.0; Cdx2tm1Fbe/Cdx2tm1Fbe (more )
Note: 16-cell embryo. Double labeled: white - Cdx2; red - Krt8 (assay MGI:3777238).
Structure Level Pattern Image Note
TS4: compacted morula Absent 5D Cdx2
Specimen 6A: embryonic day 3.0 (more )
Note: 16-cell embryo. Double labeled: white - Cdx2; red - Eomes (assay MGI:3777249).
Structure Level Pattern Image Note
TS4: compacted morula Present 6A Cdx2
Specimen 6B: embryonic day 3.5 (more )
Note: This is a blastocyst stage embryo. Double labeled: white - Cdx2; red - Eomes (assay MGI:3777249)
Structure Level Pattern Image Note
TS4: trophectoderm Present 6B Cdx2
Specimen 6E: embryonic day 3.5; Cdx2tm1Fbe/Cdx2tm1Fbe (more )
Note: This is a blastocyst stage embryo.
Structure Level Pattern Image Note
TS4: trophectoderm Absent 6E Cdx2
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory