Gene Expression Data
Assay Details
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Assay
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Reference:
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J:122362
Harwood BN, et al., Members of the WNT signaling pathways are widely expressed in mouse ovaries, oocytes, and cleavage stage embryos. Dev Dyn. 2008;237(4):1099-1111
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3775304
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Gene symbol:
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Wnt7b
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Gene name:
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wingless-type MMTV integration site family, member 7B
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Antibody:
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anti-Wnt-7a/b
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Detection system:
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Secondary antibody coupled to Alexa Fluor 555
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Assay notes:
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The antibody used in this assay recognizes both Wnt7a and Wnt7b. Analysis of staining patterns was done on at least two biological replicates.
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Results
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Specimen
3:
postnatal day 21
(more  )
Specimen
3:
(close  )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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postnatal day 21
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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3_WNT7A/B
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The inability to amplify Wnt7b in RT-PCR reactions using material derived from this stage suggests that the observed expression is likely to be due to the presence of Wnt7a protein.
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Specimen
6--FGO:
postnatal
(more )
Specimen
6--FGO:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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6_WNT7A/B FGO
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The inability to amplify Wnt7b in RT-PCR reactions using material derived from this stage suggests that the observed expression on the cell surface and in the cytoplasm of full grown oocytes is likely to be due to the presence of Wnt7a protein.
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Specimen
6--OO:
postnatal
(more )
Specimen
6--OO:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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6_WNT7A/B OO
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The inability to amplify Wnt7b in RT-PCR reactions using material derived from this stage suggests that the observed expression on the cell surface and in the cytoplasm of ovulated oocytes is likely to be due to the presence of Wnt7a protein.
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Specimen
6--Zyg:
embryonic day 0.5
(more )
Specimen
6--Zyg:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
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6_WNT7A/B Zyg
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The inability to amplify Wnt7b in RT-PCR reactions using material derived from this stage suggests that the observed expression on the cell surface and in the cytoplasm of zygotes is likely to be due to the presence of Wnt7a protein.
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Specimen
6--2C:
embryonic day 1.0
(more )
Specimen
6--2C:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
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6_WNT7A/B 2C
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In 2-cell-stage embryos expression is primarily localized on the surface with some present in the cytoplasm.
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Analysis Tools