Gene Expression Data
Assay Details
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Assay
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Reference:
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J:122362
Harwood BN, et al., Members of the WNT signaling pathways are widely expressed in mouse ovaries, oocytes, and cleavage stage embryos. Dev Dyn. 2008;237(4):1099-1111
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3775258
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Gene symbol:
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Wnt5a
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Gene name:
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wingless-type MMTV integration site family, member 5A
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Antibody:
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anti-Wnt-5a
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Detection system:
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Secondary antibody coupled to Alexa Fluor 555
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Assay notes:
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Analysis of staining patterns was done on at least two biological replicates.
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Results
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Specimen
3:
postnatal day 21
(more  )
Specimen
3:
(close  )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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postnatal day 21
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6--FGO:
postnatal
(more )
Specimen
6--FGO:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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6_WNT5A FGO
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In full grown oocytes expression is primarily localized on the cell surface with some present in the cytoplasm.
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Specimen
6--OO:
postnatal
(more )
Specimen
6--OO:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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postnatal
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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6_WNT5A OO
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In ovulated oocytes expression is primarily localized on the cell surface with some present in the cytoplasm.
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Specimen
6--Zyg:
embryonic day 0.5
(more )
Specimen
6--Zyg:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
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6_WNT5A Zyg
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In zygotes expression is primarily localized on the cell surface with some present in the cytoplasm.
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Specimen
6--2C:
embryonic day 1.0
(more )
Specimen
6--2C:
(close )
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Genetic Background:
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involves: C57BL/6J * DBA/2J
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
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6_WNT5A 2C
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In 2-cell-stage embryos expression is primarily localized on the cell surface with some present in the cytoplasm.
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Analysis Tools