Antibody:	anti-CaBP 28K
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: nephric duct, mesonephric portion	Present	Regionally restricted		Expression first observed in mesonephric duct and tubules.
TS17: mesonephric tubule	Present			Expression first observed in mesonephric duct and tubules.

Structure	Level	Pattern	Image	Note
TS19: metanephros	Present			Expression first observed in metanephric duct.

Structure	Level	Pattern	Image	Note
TS22: mesonephros	Absent			No expression in the mesonephros.

Structure	Level	Pattern	Image	Note
TS22: renal tubule	Present		3B	Expression in metanephric tubules.

Structure	Level	Pattern	Image	Note
TS23: duodenum	Present	Regionally restricted	4	Generally diffuse cytoplasmic expression in short, thick immature duodenal villi. Some expression extending into apical cytoplasmic process of an 'open type' gut endocrine cell.

Structure	Level	Pattern	Image	Note
TS25: duodenum	Present	Regionally restricted	5	Expression in mature duodenal villus, concentrated around nucleus and base of cell.

Structure	Level	Pattern	Image	Note
TS22: duodenum	Present	Regionally restricted	6	Expression in duodenum in myenteric ganglion cells and nerve fiber and in enteric nerve entering the gut wall.
TS22: enteric nervous system	Present	Regionally restricted	6	Expression in duodenum in myenteric ganglion cells and nerve fiber and in enteric nerve entering the gut wall.

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Absent			No expression in Purkinje cells.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present	Regionally restricted	9	Expression in a distinct layer of Purkinje cells in the cerebellar cortex.

Structure	Level	Pattern	Image	Note
TS28: metanephros	Present