Probe:	PRKz cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	The cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present		1A PKC z	Expression is ubiquitous; there is no tissue-specific expression at this age.
TS28: placenta	Present		1A PKC z

Structure	Level	Pattern	Image	Note
TS13: embryo	Present			Expression is ubiquitous; there is no tissue-specific expression at this age.

Structure	Level	Pattern	Image	Note
TS15: embryo	Present			Expression is ubiquitous; there is no tissue-specific expression at this age.

Structure	Level	Pattern	Image	Note
TS17: embryo	Present		1B PKC z	Expression is ubiquitous; there is no tissue-specific expression at this age.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Non-Uniform	2B	There is at least ubitquitous weak expression. Some regions have strong expression levels. One region with expression is the connective tissue bordering the intestine.
TS20: olfactory epithelium	Strong		2B , 2D
TS20: choroid plexus	Strong		2H , 2B
TS20: adenohypophysis	Strong		2B , 2J	There is strong expression in Rathke's pouch.
TS20: brain	Weak		2F , 2J , 2B , 2H	Other than in the areas noted, there is only weak expression in the brain.
TS20: liver	Weak		2B , 2L
TS20: liver sinusoid	Strong		2L , 2B
TS20: lobar bronchus	Strong		2N , 2B	There is strong expression in the segmental bronchi.
TS20: intestine epithelium	Present		2P , 2B
TS20: intestine mesenchyme	Absent		2P , 2B
TS20: stomach	Strong	Regionally restricted	2B , 2R	Expression is present in the mucous membranous lining of the stomach.
TS20: mesonephric tubule	Strong	Regionally restricted	2B , 2R	Expression is present in the epithelium.
TS20: urogenital sinus	Present		2B , 2T
TS20: urinary system	Present	Regionally restricted	2B , 2T	Expression is present in the urethra.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Regionally restricted	3B	Expression is restricted to specific tissues.
TS22: choroid plexus	Present		3B , 3F
TS22: olfactory epithelium	Present		3B
TS22: adenohypophysis	Present		3B , 3J	Expression is present in Rathke's pouch.
TS22: dorsal root ganglion	Absent		3B
TS22: spinal cord	Absent		3B
TS22: submandibular gland primordium	Absent		3B
TS22: thymus primordium	Absent		3B , 3N
TS22: cartilage	Absent		3B	There is no expression in the cartilage primoridium.
TS22: lobar bronchus	Present	Regionally restricted	3B , 3R	Expression in the lung is restricted to the terminal bronchioles and the epithelium of the bronchial tree.
TS22: maturing glomerular tuft	Present		3B , 3V	Expression in the metanephron is limited to the primitive glomeruli.
TS22: heart	Weak		3B
TS22: liver	Weak		3B

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Regionally restricted	3D	Expression is restricted to specific tissues. It is also present in the connective tissue surrounding the intestine.
TS22: choroid plexus	Present		3H , 3D
TS22: olfactory epithelium	Present		3D , 3L
TS22: adenohypophysis	Present		3D	Expression is present in Rathke's pouch.
TS22: dorsal root ganglion	Absent		3D
TS22: spinal cord	Absent		3D
TS22: submandibular gland primordium	Absent		3D
TS22: thymus primordium	Absent		3D
TS22: cartilage	Absent		3D	There is no expression in the cartilage primoridium.
TS22: intestine epithelium	Present		3T , 3X , 3D
TS22: intestine mesenchyme	Absent		3D , 3X , 3T
TS22: urogenital sinus	Strong		3T , 3D
TS22: heart	Weak		3P , 3D
TS22: liver	Weak		3D

Structure	Level	Pattern	Image	Note
TS24: choroid plexus	Present		4B , 4F
TS24: adenohypophysis	Present		4J , 4B	Expression is present in Rathke's pouch.
TS24: brown fat	Absent		4B
TS24: dorsal root ganglion	Absent		4B
TS24: spinal cord	Absent		4B
TS24: submandibular gland	Absent		4B
TS24: thymus	Absent		4B
TS24: lung	Present		4N , 4B , 4V	Expression is ubiquitous.
TS24: bronchiole epithelium	Present		4N , 4V , 4B	There is an elevated signal intensity in the epithelial cells lining the main and terminal bronchi.
TS24: renal medulla	Present		4R , 4B	In the kidney, expression is mainly restricted to the medulla.
TS24: intestine	Present		4B	Expression is found in all epithelial layers.
TS24: pigmented retinal epithelium	Present		4Y2 , 4B
TS24: presumptive iris	Present	Regionally restricted	4Y2 , 4B	Expression is present in the cuboidal epithelium.

Structure	Level	Pattern	Image	Note
TS24: choroid plexus	Present		4D
TS24: olfactory epithelium	Present		4D , 4L
TS24: adenohypophysis	Present		4D	Expression is present in Rathke's pouch.
TS24: brown fat	Absent		4D
TS24: dorsal root ganglion	Absent		4D
TS24: submandibular gland	Absent		4D
TS24: spinal cord	Absent		4H , 4D
TS24: thymus	Absent		4P , 4D
TS24: intestine	Present		4T , 4D	Expression is found in all epithelial layers.
TS24: bladder	Present		4D
TS24: seminiferous cord	Present		4D , 4X	Expression is restricted to the epithelium of the seminiferous tubuli.
TS24: skin	Present	Regionally restricted	4D , 4Z2	Expression is only found in the outermost embryonic periderm cells.

Structure	Level	Pattern	Image	Note
TS20: primitive bladder	Strong	Regionally restricted
TS20: cartilage	Weak			Expression is ubiquitous in the cartilage primordium.
TS20: choroid plexus	Strong			Expression is ubiquitous.
TS20: diencephalon	Weak			Expression is ubiquitous.
TS20: dorsal root ganglion	Trace			Expression is ubiquitous.
TS20: epidermis	Weak
TS20: heart	Weak			Expression is ubiquitous.
TS20: intestine	Strong	Regionally restricted
TS20: metanephros	Strong	Regionally restricted
TS20: liver	Present	Non-Uniform		There is at least weak expression throughout the liver; in some areas it is strong.
TS20: lung	Strong	Regionally restricted
TS20: medulla oblongata	Weak			Expression is ubiquitous.
TS20: midbrain	Weak			Expression is ubiquitous.
TS20: neocortex	Weak			Expression is ubiquitous.
TS20: olfactory epithelium	Strong			Expression is ubiquitous.
TS20: adenohypophysis	Strong			Expression is ubiquitous in Rathke's pouch.
TS20: spinal cord	Weak			Expression is ubiquitous.

Structure	Level	Pattern	Image	Note
TS22: bladder	Strong	Regionally restricted
TS22: cartilage	Absent			There is no expression in the cartilage primordium.
TS22: cerebellum	Absent
TS22: choroid plexus	Strong			Expression is ubiquitous.
TS22: diencephalon	Absent
TS22: dorsal root ganglion	Absent
TS22: epidermis	Absent
TS22: heart	Weak			Expression is ubiquitous.
TS22: intestine	Strong	Regionally restricted
TS22: metanephros	Strong	Regionally restricted
TS22: liver	Trace			Expression is ubiquitous.
TS22: lung	Present	Regionally restricted		Expression levels vary from medium to strong.
TS22: medulla oblongata	Absent
TS22: midbrain	Absent
TS22: neocortex	Absent
TS22: olfactory epithelium	Strong			Expression is ubiquitous.
TS22: pancreas	Absent
TS22: adenohypophysis	Strong			Expression is ubiquitous in Rathke's pouch.
TS22: spinal cord	Absent
TS22: submandibular gland primordium	Absent
TS22: thymus primordium	Absent

Structure	Level	Pattern	Image	Note
TS24: bladder	Strong	Regionally restricted
TS24: cartilage	Absent			There is no expression in the cartilage primordium.
TS24: cerebellum	Absent
TS24: choroid plexus	Strong			Expression is ubiquitous.
TS24: diencephalon	Absent
TS24: dorsal root ganglion	Absent
TS24: epidermis	Absent
TS24: eye	Moderate	Regionally restricted
TS24: heart	Weak			Expression is ubiquitous.
TS24: intestine	Strong	Regionally restricted
TS24: metanephros	Strong	Regionally restricted
TS24: liver	Absent
TS24: lung	Strong	Regionally restricted
TS24: medulla oblongata	Absent
TS24: midbrain	Absent
TS24: neocortex	Absent
TS24: olfactory epithelium	Strong			Expression is ubiquitous.
TS24: pancreas	Absent
TS24: adenohypophysis	Strong			Expression is ubiquitous in Rathke's pouch.
TS24: spinal cord	Absent
TS24: submandibular gland	Absent
TS24: testis	Strong	Regionally restricted
TS24: thymus	Absent			Expression is ubiquitous.