Probe:	MH2300 probe 1927
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Moderate		3B Suclg2
TS22: midbrain	Weak		3B Suclg2
TS22: cerebellum	Weak		3B Suclg2
TS22: pons	Weak		3B Suclg2
TS22: medulla oblongata	Weak		3B Suclg2
TS22: spinal cord	Weak		3B Suclg2
TS22: cranial ganglion	Weak		3B Suclg2
TS22: dorsal root ganglion	Weak		3B Suclg2
TS22: olfactory epithelium	Moderate		3B Suclg2
TS22: intestine	Strong		3B Suclg2
TS22: salivary gland	Strong		3B Suclg2
TS22: tongue	Weak		3B Suclg2
TS22: stomach	Weak		3B Suclg2
TS22: liver	Moderate		3B Suclg2
TS22: lung	Strong		3B Suclg2
TS22: metanephros	Strong		3B Suclg2
TS22: thyroid gland	Strong		3B Suclg2
TS22: pituitary gland	Strong		3B Suclg2
TS22: pancreas	Strong		3B Suclg2
TS22: adrenal gland	Strong		3B Suclg2
TS22: heart	Moderate		3B Suclg2
TS22: skeletal muscle	Weak		3B Suclg2
TS22: cartilage	Absent		3B Suclg2	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3B Suclg2	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Weak		MH2300
TS22: peripheral nervous system	Weak		MH2300
TS22: telencephalon ventricular layer	Present		MH2300	Expression is present in the ventricular zone lining the lateral ventricles of the forebrain.
TS22: telencephalon	Present	Regionally restricted	MH2300	Expression is present in the lamina terminalis.
TS22: thalamus	Present	Regionally restricted	MH2300	Expression is present in the anterior boundary of the thalamus.
TS22: pituitary gland	Present	Regionally restricted	MH2300	Expression is present in the neuronal component of the pituitary.
TS22: amygdala	Strong	Single cells	MH2300	There is strong expression in scattered cells of the amygdala.