Probe:	MH1218 probe 1006
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Moderate		3A Pklr
TS22: midbrain	Weak		3A Pklr
TS22: cerebellum	Weak		3A Pklr
TS22: pons	Weak		3A Pklr
TS22: medulla oblongata	Weak		3A Pklr
TS22: spinal cord	Weak		3A Pklr
TS22: cranial ganglion	Weak		3A Pklr
TS22: dorsal root ganglion	Weak		3A Pklr
TS22: olfactory epithelium	Moderate		3A Pklr
TS22: intestine	Strong		3A Pklr
TS22: salivary gland	Strong		3A Pklr
TS22: tongue	Weak		3A Pklr
TS22: stomach	Moderate		3A Pklr
TS22: liver	Strong		3A Pklr
TS22: lung	Strong		3A Pklr
TS22: metanephros	Moderate		3A Pklr
TS22: thyroid gland	Moderate		3A Pklr
TS22: pituitary gland	Moderate		3A Pklr
TS22: pancreas	Weak		3A Pklr
TS22: adrenal gland	Weak		3A Pklr
TS22: heart	Weak		3A Pklr
TS22: skeletal muscle	Absent		3A Pklr
TS22: cartilage	Absent		3A Pklr	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3A Pklr	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: gut	Strong		MH1218_3A
TS22: liver	Strong		MH1218_3A
TS22: lung	Strong		MH1218_3A
TS22: salivary gland	Strong		MH1218_3A
TS22: tooth	Strong		MH1218_3A
TS22: spinal cord lateral wall	Present	Regionally restricted	MH1218_3A	Expression is present in the motor column.
TS22: intervertebral disc	Present		MH1218_3A
TS22: olfactory epithelium	Present		MH1218_3A
TS22: embryo	Present		MH1218_3A	Expression is present in numerous sites, but its level is significantly lower than in the aforementioned structures.