Probe:	MH1608 probe 948
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	1M	Expression is present in the ventro-medial nucleus and the preoptic area.
TS22: adenohypophysis	Present		1M	Expression is present in the anterior pituitary.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present		1N

Structure	Level	Pattern	Image	Note
TS22: neocortex	Weak		3A Gck
TS22: midbrain	Weak		3A Gck
TS22: cerebellum	Weak		3A Gck
TS22: pons	Weak		3A Gck
TS22: medulla oblongata	Weak		3A Gck
TS22: spinal cord	Weak		3A Gck
TS22: cranial ganglion	Weak		3A Gck
TS22: dorsal root ganglion	Weak		3A Gck
TS22: olfactory epithelium	Weak		3A Gck
TS22: intestine	Weak		3A Gck
TS22: salivary gland	Weak		3A Gck
TS22: tongue	Weak		3A Gck
TS22: stomach	Weak		3A Gck
TS22: liver	Weak		3A Gck
TS22: lung	Weak		3A Gck
TS22: metanephros	Weak		3A Gck
TS22: thyroid gland	Weak		3A Gck
TS22: pituitary gland	Strong		3A Gck
TS22: pancreas	Strong		3A Gck
TS22: adrenal gland	Weak		3A Gck
TS22: heart	Weak		3A Gck
TS22: skeletal muscle	Absent		3A Gck
TS22: cartilage	Absent		3A Gck	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3A Gck	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: adenohypophysis	Present		MH1608	Expression is present in the anterior pituitary.
TS22: hypothalamus	Present	Regionally restricted	MH1608	Expression is present in the ventro-medial nucleus of the hypothalamus and in the preoptic area.
TS22: pons	Present	Regionally restricted	MH1608	Expression is present in the locus coeruleus.
TS22: pancreas	Present		MH1608