Gene Expression Data
Assay Details
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Assay
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Reference:
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J:112741
Gagnon LH, et al., The chloride intracellular channel protein CLIC5 is expressed at high levels in hair cell stereocilia and is essential for normal inner ear function. J Neurosci. 2006 Oct 4;26(40):10188-98
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3769024
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Gene symbol:
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Clic5
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Gene name:
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chloride intracellular channel 5
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Antibody:
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CLIC5 antibody
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
7A:
embryonic day 16.5
(more  )
Specimen
7A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cochlea |
Present |
Regionally restricted |
7A
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Expression is localized to microvilli-covered apical surfaces of interdental cells, columnar cells of Kolliker's organ, and to stereocilia of inner and outer hair cells.
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Specimen
7B:
postnatal day 1
(more )
Specimen
7B:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Present |
Regionally restricted |
7B
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Expression is localized to microvilli-covered apical surfaces of interdental cells, columnar cells of Kolliker's organ, and to stereocilia of inner and outer hair cells.
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Specimen
7C:
(close )
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Genetic Background:
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C3H/HeJ-Clic5jbg/J
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Mutant Allele(s):
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Clic5jbg/Clic5jbg
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7D:
postnatal day 6
(more )
Specimen
7D:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
Present |
Regionally restricted |
7D
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Expression is localized to microvilli-covered apical surfaces of interdental cells, columnar cells of Kolliker's organ, and to stereocilia of inner and outer hair cells.
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Specimen
7E:
postnatal day 15
(more )
Specimen
7E:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlea |
Strong |
Regionally restricted |
7E
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Immunofluorescence was most intense in hair cell stereocilia.
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Specimen
7F:
postnatal day 1
(more )
Specimen
7F:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: utricle |
Present |
Regionally restricted |
7F
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Expression is in stereocilia of vestibular hair cells, in the cristae of the semicircular canals.
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Specimen
7G:
postnatal day 6
(more )
Specimen
7G:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: utricle |
Present |
Regionally restricted |
7G
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Expression is in stereocilia of vestibular hair cells, in the cristae of the semicircular canals.
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Specimen
7H:
postnatal day 1
(more )
Specimen
7H:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7I:
postnatal day 6
(more )
Specimen
7I:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7J:
postnatal day 15
(more )
Specimen
7J:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7K:
(close )
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Genetic Background:
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C3H/HeJ-Clic5jbg/J
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Mutant Allele(s):
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Clic5jbg/Clic5jbg
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7L:
postnatal day 30
(more )
Specimen
7L:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal day 30
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Not shown:
(close )
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Genetic Background:
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C3H/HeJ-Clic5jbg/J
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Mutant Allele(s):
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Clic5jbg/Clic5jbg
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Not shown:
(close )
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Genetic Background:
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C3H/HeJ-Clic5jbg/J
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Mutant Allele(s):
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Clic5jbg/Clic5jbg
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Not shown:
(close )
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Genetic Background:
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C3H/HeJ-Clic5jbg/J
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Mutant Allele(s):
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Clic5jbg/Clic5jbg
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools