Trip13 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:123677 Li X, et al., Mouse Pachytene Checkpoint 2 (Trip13) Is Required for Completing Meiotic Recombination but Not Synapsis. PLoS Genet. 2007 Aug 10;3(8):e130
Assay type:	Western blot
MGI Accession ID:	MGI:3769018
Gene symbol:	Trip13
Gene name:	thyroid hormone receptor interactor 13

Antibody:	anti-TRIP13
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	48 kDa	38 kDa	Genetic Background	Mutant Allele(s)	Sex
+/+	P adult	TS28: testis	Present (a)	Present	Not Specified		Male
Het	P adult	TS28: testis	Present (a)	Present	involves: 129P2/OlaHsd * C57BL/6	Trip13^{Gt(RRB047)Byg}/Trip13⁺	Male
Mut	P adult	TS28: testis	Absent	Weak (b)	involves: 129P2/OlaHsd * C57BL/6	Trip13^{Gt(RRB047)Byg}/Trip13^{Gt(RRB047)Byg}	Male

Notes:
(a) Authors report multiple species in wild type and heterozygous testis, one of which corresponds to the expected size of 48 kDa.
(b) A reduced amount of a smaller 38 kDa band was present in homozygous mutant testis. Authors state that it is not clear if this corresponds to TRIP13.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24