Trip13 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:123677 Li X, et al., Mouse Pachytene Checkpoint 2 (Trip13) Is Required for Completing Meiotic Recombination but Not Synapsis. PLoS Genet. 2007 Aug 10;3(8):e130
Assay type:	Western blot
MGI Accession ID:	MGI:3769018
Gene symbol:	Trip13
Gene name:	thyroid hormone receptor interactor 13

Antibody:	anti-TRIP13
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	48 kDa	38 kDa	Genetic Background	Mutant Allele(s)	Sex
+/+	P adult	TS28: testis	Present (a)	Present	Not Specified		Male
Het	P adult	TS28: testis	Present (a)	Present	involves: 129P2/OlaHsd * C57BL/6	Trip13^{Gt(RRB047)Byg}/Trip13⁺	Male
Mut	P adult	TS28: testis	Absent	Weak (b)	involves: 129P2/OlaHsd * C57BL/6	Trip13^{Gt(RRB047)Byg}/Trip13^{Gt(RRB047)Byg}	Male

Notes:
(a) Authors report multiple species in wild type and heterozygous testis, one of which corresponds to the expected size of 48 kDa.
(b) A reduced amount of a smaller 38 kDa band was present in homozygous mutant testis. Authors state that it is not clear if this corresponds to TRIP13.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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