Probe:	Emx2 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		2f	Expression in presumptive cortex.
TS20: telencephalon	Present	Regionally restricted	2f	Much stronger expression in posterior dorsal telencephalon, with a sharp posterior boundary and decreasingly intensity in progressively more anterior regions.
TS20: head	Present	Regionally restricted	2f	Expression in ectodermal regions in the snout.
TS20: reproductive system	Present	Regionally restricted	2f	Expression in the genital ridge.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		2g	Expression in presumptive cortex.
TS20: telencephalon	Present	Regionally restricted	2g	Much stronger expression in posterior dorsal telencephalon, with a sharp posterior boundary and decreasingly intensity in progressively more anterior regions.
TS20: midbrain	Present	Regionally restricted	2g	Expression in limited mesencephalic areas.
TS20: nasal cavity epithelium	Present		2g
TS20: head	Present	Regionally restricted	2g	Expression in ectodermal regions in the snout.
TS20: reproductive system	Present	Regionally restricted	2g	Expression in the genital ridge.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		2h	Expression in presumptive cortex.
TS20: telencephalon	Present	Regionally restricted	2h	Much stronger expression in posterior dorsal telencephalon, with a sharp posterior boundary and decreasingly intensity in progressively more anterior regions.
TS20: diencephalon	Present	Regionally restricted	2h	Expression domain in the head appears to be extended to specific diencephalic regions.
TS20: thalamus	Present	Regionally restricted	2h	Diffuse expression in the ventral thalamus.
TS20: midbrain	Present	Regionally restricted	2h	Expression in limited mesencephalic areas.
TS20: nasal cavity epithelium	Present		2h
TS20: head	Present	Regionally restricted	2h	Expression in ectodermal regions in the snout.
TS20: metanephros	Present	Regionally restricted	2h	Expression in metanephric tubular epithelia.
TS20: reproductive system	Present	Regionally restricted	2h	Expression in the genital ridge.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		2i	Expression in presumptive cortex.
TS20: telencephalon	Present	Regionally restricted	2i	Much stronger expression in posterior dorsal telencephalon, with a sharp posterior boundary and decreasingly intensity in progressively more anterior regions. Expression in the olfactory bulbs.
TS20: diencephalon	Present	Regionally restricted	2i	Expression domain in the head appears to be extended to specific diencephalic regions.
TS20: thalamus	Present	Regionally restricted	2i	Diffuse expression in the ventral thalamus.
TS20: midbrain	Present	Regionally restricted	2i	Expression in limited mesencephalic areas.
TS20: nasal cavity epithelium	Present		2i
TS20: head	Present	Regionally restricted	2i	Expression in ectodermal regions in the snout.
TS20: metanephros	Present	Regionally restricted	2i	Expression in metanephric tubular epithelia.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		2j	Expression in presumptive cortex.
TS20: telencephalon	Present	Regionally restricted	2j	Much stronger expression in posterior dorsal telencephalon, with a sharp posterior boundary and decreasingly intensity in progressively more anterior regions.
TS20: diencephalon	Present	Regionally restricted	2j	Expression domain in the head appears to be extended to specific diencephalic regions.
TS20: thalamus	Present	Regionally restricted	2j	Diffuse expression in the ventral thalamus.
TS20: midbrain	Present	Regionally restricted	2j	Expression in limited mesencephalic areas.
TS20: head	Present	Regionally restricted	2j	vExpression in ectodermal regions in the snout.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	2l	Expression is not uniformly distributed across the cortical layers
TS20: cerebral cortex mantle layer	Trace		2l	Almost undetectable within the external mantle layer.
TS20: telencephalon	Present	Regionally restricted	2l	No expression detectable in the internal basal telencephalic regions.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		2m
TS20: nasal cavity respiratory epithelium	Absent		2m

Structure	Level	Pattern	Image	Note
TS21: nasal cavity	Present		2p
TS21: diencephalon	Present	Regionally restricted	2p	Expression in mammillary recess
TS21: head	Present	Regionally restricted	2p	Expression in ectodermal regions in the snout and limbs.
TS21: limb	Present	Regionally restricted	2p	Expression in ectodermal regions in the snout and limbs.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex	Present	Regionally restricted	2q	Uneven localization of expression across the cortical layers.
TS21: diencephalon	Present	Regionally restricted	2q	Localized expression in the diencephalon. Expression in mammillary recess.
TS21: renal tubule	Present	Regionally restricted	2q	Expression in metanephric tubular epithelia.

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	3e	Diverse expression in the head.
TS20: midbrain	Present	Regionally restricted	3e	Localized expression in mesencephalic areas.

Structure	Level	Pattern	Image	Note
TS20: head	Present	Regionally restricted	3f	Diverse expression in the head.
TS20: midbrain	Present	Regionally restricted	3f	Localized expression in mesencephalic areas. Possible expression in interpeduncular areas.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3g	Expression intensity in the diencephalon varies with the anteroposterior level of the section.
TS20: thalamus	Present	Regionally restricted	3g	Thalamic expression is strong in a limited region of the ventral portion of the dorsal thalamus.
TS20: hypothalamus	Present	Regionally restricted	3g	Expression in hypothalamic regions just posterior to the localization of Rathke's pouch.
TS20: head	Present	Regionally restricted	3g	Authors report expression in branchial arches in the first, mandibular, arch

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3h	Expression intensity in the diencephalon varies with the anteroposterior level of the section.
TS20: thalamus	Present	Regionally restricted	3h	Thalamic expression is strong in a limited region of the ventral portion of the dorsal thalamus.
TS20: epithalamus	Present		3h
TS20: head	Present	Regionally restricted	3h	Authors report expression in branchial arches in the first, mandibular, arch.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	3i	Expression intensity in the diencephalon varies with the anteroposterior level of the section.
TS20: thalamus	Present	Regionally restricted	3i	Thalamic expression is strong in a limited region of the ventral portion of the dorsal thalamus.
TS20: head	Present	Regionally restricted	3i	Authors report expression in branchial arches in the first, mandibular, arch.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	3j	Expression in dorsal and posterior basal cortical regions.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	3k	Expression in dorsal and posterior basal cortical regions.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	3l	Expression in dorsal and posterior basal cortical regions.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		3p	Major site of expression is in the cortex.
TS20: diencephalon	Present		3p

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		3q	Major site of expression is in the cortex.
TS20: diencephalon	Present		3q

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		3r	Major site of expression is in the cortex.
TS20: diencephalon	Present		3r
TS20: midbrain	Present		3r

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	4c	Expression in anterior dorsal neurectodermal regions.

Structure	Level	Pattern	Image	Note
TS14: central nervous system	Present	Regionally restricted	4d	Authors report stronger expression in dorsal neurectoderm.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	4e	Clear antero-posterior delimination of expression in dorsal neurectoderm.

Structure	Level	Pattern	Image	Note
TS16: future forebrain	Present	Regionally restricted	4f	Clear antero-posterior delimination of expression in dorsal neurectoderm, with a posterior boundary well within the roof of the presumptive diencephalon.
TS16: diencephalon	Present	Regionally restricted	4f	Ventral expression in the floor of the presumptive diencephalon.
TS16: future midbrain	Absent		4f
TS16: surface ectoderm	Present	Regionally restricted	4f	Anterior ectodermal localization of expression, in a continuous region probably including the olfactory placodes.
TS16: genitourinary system	Present	Regionally restricted	4f	Expression in coelomic epithelium covering the mesonephric column, as well as the final part of the mesenteric attachment, fated to contribute to the genital ridge.

Structure	Level	Pattern	Image	Note
TS23: embryo	Present	Single cells		Authors report expression patterns identical to that seen in E13.5 embryos.

Structure	Level	Pattern	Image	Note
TS25: head	Present	Regionally restricted	5f	Authors state that expression reproduces that of Emx1.
TS25: olfactory epithelium	Present		5f

Structure	Level	Pattern	Image	Note
TS25: head	Present	Regionally restricted	5g	Authors state that expression reproduces that of Emx1.

Structure	Level	Pattern	Image	Note
TS25: head	Present	Regionally restricted	5h	Authors state that expression reproduces that of Emx1.

Structure	Level	Pattern	Image	Note
TS25: head	Present	Regionally restricted	5i	Authors state that expression reproduces that of Emx1.

Structure	Level	Pattern	Image	Note
TS25: head	Present	Regionally restricted	5j	Authors state that expression reproduces that of Emx1.
TS25: hypothalamus	Present	Regionally restricted	5j	Expression in posterior hypothalamic germinal areas.

Structure	Level	Pattern	Image	Note
TS25: head	Present	Regionally restricted	5l	Authors state that expression reproduces that of Emx1.

Structure	Level	Pattern	Image	Note
TS21: nasal cavity	Present		5m

Structure	Level	Pattern	Image	Note
TS25: head	Present	Regionally restricted	5n	Authors state that expression reproduces that of Emx1.