Probe:	FG36 probe 23
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Absent		3C

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Absent		3G

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Present		3K	Expression in present in the proliferating chondrocytes in the core region of the ribs.

Structure	Level	Pattern	Image	Note
TS22: intercostal muscle	Absent		3O	There is no expression in the intercostal muscle fibers attached to the sternum.

Structure	Level	Pattern	Image	Note
TS22: brain ventricular layer	Present	Non-Uniform	FG36	There are gaps in the staining in the ventricular zone.
TS22: corpus striatum	Present	Single cells	FG36	Expression is present in numerous scattered neurons.
TS22: medulla oblongata	Present	Single cells	FG36	Expression is present in numerous scattered neurons.
TS22: midbrain	Present	Single cells	FG36	Expression is present in numerous scattered neurons.
TS22: pons	Present	Single cells	FG36	Expression is present in numerous scattered neurons.
TS22: spinal cord ventricular layer	Strong		FG36
TS22: spinal cord	Present		FG36	There is expression in cells surrounding the ventricular zone.
TS22: choroid plexus	Absent		FG36
TS22: cortical plate	Absent		FG36
TS22: hippocampus	Absent		FG36
TS22: hypothalamus	Present	Single cells	FG36	There are a few scattered neurons.
TS22: endocardial tissue	Present		FG36
TS22: intestine epithelium	Present		FG36
TS22: lens	Present		FG36
TS22: lung epithelium	Present		FG36
TS22: lung mesenchyme	Present		FG36
TS22: pancreas	Present		FG36
TS22: skeleton	Present		FG36	Expression is present in the skeletal primordium.
TS22: metanephros	Weak		FG36