Probe:	FG35 probe 22
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Absent		3B

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Present		3F

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Absent		3J	There is no expression in the proliferating chondrocytes in the core region of the ribs.

Structure	Level	Pattern	Image	Note
TS22: intercostal muscle	Absent		3N	There is no expression in the intercostal muscle fibers attached to the sternum.

Structure	Level	Pattern	Image	Note
TS22: brain ventricular layer	Strong	Non-Uniform	FG35	Expression in the ventricular zone varies locally; in some regions it is strong.
TS22: spinal cord ventricular layer	Strong	Non-Uniform	FG35	Expression in the ventricular zone varies locally; in some regions it is strong.
TS22: choroid plexus	Strong		FG35
TS22: cortical plate	Absent		FG35
TS22: hippocampus	Trace		FG35	Expression levels are near background.
TS22: brainstem	Present	Single cells	FG35	Expression is present in numerous scattered neuronal precursors.
TS22: midbrain	Present	Single cells	FG35	Expression is present in numerous scattered neuronal precursors.
TS22: pons	Present	Single cells	FG35	Expression is present in numerous scattered neuronal precursors.
TS22: spinal cord	Present	Non-Uniform	FG35	There is strong expression in the roof, as well as in neurons elsewhere.
TS22: epithalamus	Present	Regionally restricted	FG35	Expression is present in the pineal gland primordium.
TS22: pituitary gland	Present		FG35
TS22: hypothalamus	Present	Regionally restricted	FG35	Expression is present in various regions of the anterior hypothalamus.
TS22: adrenal gland	Present		FG35
TS22: appendicular skeleton	Present		FG35
TS22: axial skeleton	Present		FG35
TS22: gut	Present		FG35
TS22: heart valve	Present		FG35
TS22: lung	Present		FG35
TS22: metanephros	Present		FG35
TS22: pancreas	Present		FG35