Probe:	FG34 probe 21
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present	Regionally restricted	3A	Expression is present in a subset of neurons.

Structure	Level	Pattern	Image	Note
TS22: thymus primordium	Absent		3E

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Absent		3I	There is no expression in the proliferating chondrocytes in the core region of the ribs.

Structure	Level	Pattern	Image	Note
TS22: intercostal muscle	Absent		3M	There is no expression in the intercostal muscle fibers attached to the sternum.

Structure	Level	Pattern	Image	Note
TS22: brain ventricular layer	Strong		FG34	There are high levels of transcript throughout the ventricular zone, including that of the spinal cord.
TS22: spinal cord ventricular layer	Strong		FG34	There are high levels of transcript throughout the ventricular zone, including that of the spinal cord.
TS22: brainstem	Present	Single cells	FG34	There are scattered expressing cells in several brain regions.
TS22: corpus striatum	Present	Single cells	FG34	There are scattered expressing cells in several brain regions.
TS22: pons	Present	Single cells	FG34	There are scattered expressing cells in several brain regions.
TS22: spinal cord	Present	Single cells	FG34	There are scattered expressing cells in several brain regions.
TS22: hippocampus	Strong		FG34
TS22: cortical plate	Present		FG34
TS22: embryo	Present		FG34	Expression is present in many tissues of mesodermal and mesenchymal origin.