Gene Expression Data
Assay Details
Assay
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Reference:
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J:1461
Simeone A, et al., Two vertebrate homeobox genes related to the Drosophila empty spiracles gene are expressed in the embryonic cerebral cortex. EMBO J. 1992 Jul;11(7):2541-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3766945
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Gene symbol:
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Emx1
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Gene name:
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empty spiracles homeobox 1
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Probe:
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Emx1 cDNA
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2a:
embryonic day 12.5
(more )
Specimen
2a:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
|
2a
|
Expression in presumptive cortex from posterior to anterior boundary
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TS20: telencephalon |
Present |
Regionally restricted |
2a
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No expression in non-cortical basal telencephalic regions.
|
Specimen
2b:
embryonic day 12.5
(more )
Specimen
2b:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
|
2b
|
Expression in presumptive cortex from posterior to anterior boundary.
|
TS20: telencephalon |
Present |
Regionally restricted |
2b
|
No expression in non-cortical basal telencephalic regions.
|
Specimen
2c:
embryonic day 12.5
(more )
Specimen
2c:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
|
2c
|
Expression in presumptive cortex from posterior to anterior boundary.
|
TS20: telencephalon |
Present |
Regionally restricted |
2c
|
No expression in non-cortical basal telencephalic regions.
|
Specimen
2d:
embryonic day 12.5
(more )
Specimen
2d:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
|
2d
|
Expression in presumptive cortex from posterior to anterior boundary.
|
TS20: telencephalon |
Present |
Regionally restricted |
2d
|
Expression in the olfactory bulb. No expression in non-cortical basal telencephalic regions.
|
Specimen
2e:
embryonic day 12.5
(more )
Specimen
2e:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
|
2e
|
Expression in presumptive cortex from posterior to anterior boundary.
|
TS20: telencephalon |
Present |
Regionally restricted |
2e
|
No expression in non-cortical basal telencephalic regions.
|
Specimen
2k:
embryonic day 12.5
(more )
Specimen
2k:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Specimen
2n:
embryonic day 13.5
(more )
Specimen
2n:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Present |
|
2n
|
Expression is similar to that seen at E12.5
|
TS21: telencephalon |
Present |
Regionally restricted |
2n
|
Authors report a single spot of non-cortical expression, probably corresponding to a periventricular complex at the posterior boundary of the diencephalon.
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Specimen
2o:
embryonic day 13.5
(more )
Specimen
2o:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Present |
|
2o
|
Expression is similar to that seen at E12.5
|
TS21: telencephalon |
Present |
Regionally restricted |
2o
|
Authors report a single spot of non-cortical expression, probably corresponding to a periventricular complex at the posterior boundary of the diencephalon.
|
Specimen
3a:
embryonic day 12.5
(more )
Specimen
3a:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3b:
embryonic day 12.5
(more )
Specimen
3b:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3c:
embryonic day 12.5
(more )
Specimen
3c:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3d:
embryonic day 12.5
(more )
Specimen
3d:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3m:
embryonic day 12.5
(more )
Specimen
3m:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
|
3m
|
|
TS20: telencephalon |
Present |
Regionally restricted |
3m
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Restricted expression in dorsal regions of lateral ventricles including hippocampal and parahippocampal areas
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Specimen
3n:
embryonic day 12.5
(more )
Specimen
3n:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebral cortex |
Present |
|
3n
|
|
TS20: telencephalon |
Present |
Regionally restricted |
3n
|
Restricted expression in dorsal regions of lateral ventricles including hippocampal and parahippocampal areas
|
Specimen
3o:
embryonic day 12.5
(more )
Specimen
3o:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
4a:
embryonic day 9.5
(more )
Note: 22-23 somites.
Specimen
4a:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 9.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Note:
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22-23 somites.
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Specimen
4b:
embryonic day 9.75
(more )
Note: 27 somites.
Specimen
4b:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 9.75
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
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27 somites.
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: future brain |
Present |
Regionally restricted |
4b
|
Antero-posterior delimitation of expression domain is more evident, with a posterior boundary probably coinciding with that between presumptive diencephalon and telencephalon.
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TS16: branchial arch ectoderm |
Absent |
|
4b
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Specimen
not shown:
embryonic day 15.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: embryo |
Present |
Regionally restricted |
|
Authors report expression patterns identical to that seen in E13.5 embryos.
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Specimen
5a:
embryonic day 17.0
(more )
Specimen
5a:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 17.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5b:
embryonic day 17.0
(more )
Specimen
5b:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: head |
Present |
Regionally restricted |
5b
|
|
Specimen
5c:
embryonic day 17.0
(more )
Specimen
5c:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5d:
embryonic day 17.0
(more )
Specimen
5d:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: head |
Present |
Regionally restricted |
5d
|
|
TS25: cerebral cortex |
Present |
Regionally restricted |
5d
|
Reduced cortical expression. Expression in primordium of the amigdaloid cortical complex.
|
TS25: hippocampus |
Present |
Regionally restricted |
5d
|
Expression in hippocampal germinal layer.
|
Specimen
5e:
embryonic day 17.0
(more )
Specimen
5e:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5k:
embryonic day 17.0
(more )
Specimen
5k:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 17.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: head |
Present |
Regionally restricted |
5k
|
|
TS25: cerebral cortex |
Present |
Regionally restricted |
5k
|
Reduced cortical expression. Expression in primordium of the amigdaloid cortical complex.
|
TS25: hippocampus |
Present |
Regionally restricted |
5k
|
Expression in hippocampal germinal layer.
|
|