Gene Expression Data
Assay Details
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Assay
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Reference:
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J:85300
Helmbacher F, et al., Met signaling is required for recruitment of motor neurons to PEA3-positive motor pools. Neuron. 2003 Aug 28;39(5):767-77
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3763260
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Gene symbol:
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Ret
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Gene name:
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ret proto-oncogene
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Probe:
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Ret probe1
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Probe preparation:
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labelled with digoxigenin RNA
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Results
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Specimen
S1E:
embryonic day 12.5
(more )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The specimen was a flat-mounted spinal cord.
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Specimen
S1F:
embryonic day 12.5; Mettm1Cpo/ Mettm1Cpo
(more )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The specimen was a flat-mounted spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
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S1F
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No changes in expression level were detected in the mutant.
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Specimen
S1K:
embryonic day 14.5
(more )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1K:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The specimen was a flat-mounted spinal cord.
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Specimen
S1L:
embryonic day 14.5; Mettm1Cpo/ Mettm1Cpo
(more )
Note: The specimen was a flat-mounted spinal cord.
Specimen
S1L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The specimen was a flat-mounted spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: spinal cord |
Weak |
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S1L
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Expression was dramatically reduced in the lateral motor columns of mutant spinal cords, but expression in the medial motor column motor neurons was unaltered.
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