Gene Expression Data
Assay Details
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Assay
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Reference:
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J:85300
Helmbacher F, et al., Met signaling is required for recruitment of motor neurons to PEA3-positive motor pools. Neuron. 2003 Aug 28;39(5):767-77
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3763245
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Gene symbol:
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Etv4
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Gene name:
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ets variant 4
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Embryos were postfixed in 4% paraformaldehyde after X-gal staining and embedded in gelatin/Albumin before vibratome cross-sections were collected.
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Results
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Specimen
3A 40s:
embryonic day 11.0; Etv4tm1Arbr/Etv4 +
(more  )
Note: 40 somites. The specimen was a flat-mounted spinal cord.
Specimen
3A 40s:
(close  )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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40 somites. The specimen was a flat-mounted spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
3A
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Expression was restricted to the C7-C8 region of the spinal cord.
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Specimen
3B 41s:
embryonic day 11.0; Etv4tm1Arbr/Etv4 +
(more )
Note: 41 somites. The specimen was a flat-mounted spinal cord.
Specimen
3B 41s:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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41 somites. The specimen was a flat-mounted spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
3B
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Expression was restricted to the C7-C8 region of the spinal cord.
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Specimen
3C 43s:
embryonic day 11.0; Etv4tm1Arbr/Etv4 +
(more )
Note: 43 somites. The specimen was a flat-mounted spinal cord.
Specimen
3C 43s:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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43 somites. The specimen was a flat-mounted spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
3C
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Expression in the C7-C8 region of the spinal cord was starting to spread anteriorly at the 43 somite stage.
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Specimen
3D 58s:
embryonic day 12.5; Etv4tm1Arbr/Etv4 +
(more )
Note: 58 somites. The specimen was a flat-mounted spinal cord.
Specimen
3D 58s:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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58 somites. The specimen was a flat-mounted spinal cord.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Present |
Regionally restricted |
3D
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Expression was at its maximal extent and spanned the C5-C8 region of the spinal cord.
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Specimen
3E 39s:
embryonic day 10.5; Etv4tm1Arbr/Etv4 +
(more )
Note: 39 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
Specimen
3E 39s:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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39 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
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Expression was detected in the C7-C8 region of the spinal cord.
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Specimen
3E 39s:
embryonic day 10.5; Etv4tm1Arbr/Etv4 +, Mettm1Cpo/ Mettm1Cpo
(more )
Note: 39 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
Specimen
3E 39s:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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39 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: future spinal cord |
Present |
Regionally restricted |
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Normal expression was detected in the C7-C8 region of the spinal cord of mutant embryos.
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Specimen
3E 40s:
embryonic day 11.0; Etv4tm1Arbr/Etv4 +
(more )
Note: 40 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
Specimen
3E 40s:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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40 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
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Expression was detected in the C7-C8 region of the spinal cord.
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Specimen
3E 40s:
embryonic day 11.0; Etv4tm1Arbr/Etv4 +, Mettm1Cpo/ Mettm1Cpo
(more )
Note: 40 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
Specimen
3E 40s:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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40 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
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Normal expression was detected in the C7-C8 region of the spinal cord of mutant embryos.
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Specimen
3E 41s:
embryonic day 11.0; Etv4tm1Arbr/Etv4 +
(more )
Note: 41 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
Specimen
3E 41s:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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41 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
|
Expression was detected in the C7-C8 region of the spinal cord.
|
Specimen
3E 41s:
embryonic day 11.0; Etv4tm1Arbr/Etv4 +, Mettm1Cpo/ Mettm1Cpo
(more )
Note: 41 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
Specimen
3E 41s:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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41 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
Regionally restricted |
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Normal expression was detected in the C7-C8 region of the spinal cord of mutant embryos.
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Specimen
3E 43-45s:
embryonic day 11.0; Etv4tm1Arbr/Etv4 +
(more )
Note: 43-45 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
Specimen
3E 43-45s:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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43-45 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
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Specimen
3E 43-45s:
embryonic day 11.0; Etv4tm1Arbr/Etv4 +, Mettm1Cpo/ Mettm1Cpo
(more )
Note: 43-45 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
Specimen
3E 43-45s:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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43-45 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: future spinal cord |
Present |
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There were significantly fewer positive neurons in the mutant spinal cord.
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Specimen
3E 47-48s:
embryonic day 11.5; Etv4tm1Arbr/Etv4 +
(more )
Note: 47-48 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
Specimen
3E 47-48s:
(close )
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Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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47-48 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
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Specimen
3E 47-48s:
embryonic day 11.5; Etv4tm1Arbr/Etv4 +, Mettm1Cpo/ Mettm1Cpo
(more )
Note: 47-48 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
Specimen
3E 47-48s:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4+
Mettm1Cpo/Mettm1Cpo
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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47-48 somites. The data in Figure 3E was presented graphically as counts of the number of B-galactosidase-positive motor neurons.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: future spinal cord |
Present |
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There were significantly fewer positive neurons in the mutant spinal cord.
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