Probe:	Extl3 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Fixative was 10% formaline/70% methanol

Structure	Level	Pattern	Image	Note
TS19: hindbrain ventricular layer	Strong		1A
TS19: mesencephalic vesicle	Strong		1A
TS19: telencephalon ventricular layer	Strong		1A

Structure	Level	Pattern	Image	Note
TS20: ganglionic eminence	Strong		1C
TS20: medulla oblongata ventricular layer	Strong		1C
TS20: telencephalon ventricular layer	Strong		1C
TS20: vestibulo-cochlear ganglion	Present		1C

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Strong		1D
TS20: dorsal root ganglion	Present		1D

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		1E
TS22: hindbrain ventricular layer	Present		1E
TS22: midbrain ventricular layer	Present		1E
TS22: cerebellum	Weak		1E
TS22: hypothalamus	Weak		1E
TS22: thalamus	Weak		1E

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Strong		1F
TS24: hypothalamus	Weak		1F
TS24: telencephalon ventricular layer	Weak		1F
TS24: thalamus	Weak		1F

Structure	Level	Pattern	Image	Note
TS19: telencephalon ventricular layer	Very strong		2A	Expression is in most cells.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Strong		2B
TS20: cerebral cortex ventricular layer	Strong		2B
TS20: cortical plate	Present	Single cells	2B	A moderate number of heavily stained cells appear among many lightly stained or negative cells in the preplate.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Strong		2C	Expression is in mitotic cells.

Structure	Level	Pattern	Image	Note
TS20: cortical plate	Present	Single cells	2D	A moderate number of heavily stained cells appear among many lightly stained or negative cells in the preplate.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present	Uniform	2E
TS22: cerebral cortex ventricular layer	Present	Uniform	2E
TS22: cortical plate	Present	Uniform	2E

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Very strong		2F
TS24: cerebral cortex intermediate zone	Weak		2F
TS24: cerebral cortex ventricular layer	Weak		2F

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Strong	Spotted	2G	There are a large number of heavily stained cells among moderate number of less intensely stained and some negatively stained populations of cells.

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Strong	Spotted	2H	There are a large number of heavily stained cells among moderate number of less intensely stained and some negatively stained populations of cells.

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Strong		3A
TS28: cortical layer I	Moderate		3A
TS28: cortical layer II	Moderate		3A
TS28: cortical layer III	Moderate		3A
TS28: cortical layer IV	Moderate		3A
TS28: cortical layer VI	Moderate		3A

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Strong	Single cells	3C	The pyramidal neurons are showing the strongest color reaction while the granule-type neurons are showing considerably less intense signal. Absent in glial cells.

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Very strong	Single cells	3D	Expression is in the giant pyramidal cells of Betz, the apical dendrites of these cells are also showing intense positive reaction.

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Strong		3E
TS28: hippocampus granule cell layer	Present		3E
TS28: dentate gyrus polymorphic layer	Present		3E

Structure	Level	Pattern	Image	Note
TS28: hippocampus pyramidal cell layer	Strong		3F
TS28: dentate gyrus polymorphic layer	Present		3F
TS28: hippocampus granule cell layer	Present		3F
TS28: choroid plexus	Present	Regionally restricted	3F	Expression is in the choroid plexus of the inferior horn of the lateral ventricle.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Strong		3G
TS28: main olfactory bulb glomerular layer	Present	Regionally restricted	3G	Expression is in most neurons.
TS28: main olfactory bulb granule cell layer	Present	Regionally restricted	3G	Expression is in most neurons.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb mitral cell layer	Strong		3H
TS28: main olfactory bulb glomerular layer	Present		3H	Expression is in most neurons.
TS28: main olfactory bulb granule cell layer	Present		3H	Expression is in most neurons.

Structure	Level	Pattern	Image	Note
TS28: basal ganglia	Present			Expression is in most neurons.
TS28: thalamus	Present			Expression is in most neurons.