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Gene Expression Data
Assay Details
Assay
Reference: J:64068 Lu J, et al., The basic helix-loop-helix transcription factor capsulin controls spleen organogenesis. Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9525-30
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:3761161
Gene symbol: Tcf21
Gene name: transcription factor 21
Reporter: lacZ
Direct Detection
Results
Specimen Not shown: embryonic day 8.5; Tcf21tm2Eno/Tcf21tm2Eno (more )
Structure Level Pattern Image Note
TS13: 1st branchial arch mesenchyme Present Regionally restricted Expression is present in mesodermal cells migrating into the arch.
TS13: 2nd branchial arch mesenchyme Present Regionally restricted Expression is present in mesodermal cells migrating into the arch.
Specimen 3A: embryonic day 9.5; Tcf21tm2Eno/Tcf21tm2Eno (more )
Structure Level Pattern Image Note
TS15: paraxial mesenchyme Present 3A Expression is present in skeletal muscle precursors.
TS15: pericardial component Present Regionally restricted 3A Expression is present in the proepicardial organ.
Specimen 3B: embryonic day 9.5; Tcf21tm2Eno/Tcf21tm2Eno (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS15: paraxial mesenchyme Present Regionally restricted 3B Expression is present in a core of mesenchymal cells adjacent to the branchial arch arteries. These cells appear to correspond to skeletal muscle precursors.
Specimen 3C: embryonic day 10.5; Tcf21tm2Eno/Tcf21tm2Eno (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS18: pericardio-peritoneal canal mesothelium Present 3C Expression is present in a bilaterally symmetrical pattern in the mesothelium lining the visceral and parietal aspects of the pericardio-peritoneal canals.
TS18: septum transversum Strong Regionally restricted 3C There is strong expression in the hepatic primordium within the septum tranversum.
Specimen 3D: embryonic day 10.5; Tcf21tm2Eno/Tcf21tm2Eno (more )
Note: This is a sagittal section.
Structure Level Pattern Image Note
TS18: visceral pericardium Present 3D Expression is present in the proepicardial organ and epicardium.
Specimen 3E: embryonic day 13.5; Tcf21tm2Eno/Tcf21tm2Eno (more )
Structure Level Pattern Image Note
TS21: skeletal muscle Present Regionally restricted 3E Expression is present in the jaw muscles.
TS21: tongue Present 3E
TS21: visceral pericardium Present 3E
TS21: esophagus mesenchyme Present 3E Expression is present in the smooth muscle layers.
TS21: intestine mesenchyme Present Regionally restricted 3E Expression is present in the smooth muscle layers of the gastrointestinal tract.
TS21: reproductive system Present Regionally restricted 3E Expression is present in the smooth muscle layers of the urogenital tract.
Specimen 3F: embryonic day 10.5; Tcf21tm2Eno/Tcf21tm2Eno (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS18: pericardio-peritoneal canal mesothelium Present Regionally restricted 3F Expression is present on the left side of the embryo in the visceral mesothelium.
TS18: trunk mesenchyme Present Regionally restricted 3F Expression is present on the left side of the embryo in mesoderm adjacent to the visceral mesothelium. (This mesoderm is believed to be the splenic anlage.)
Specimen 3G: embryonic day 11.5; Tcf21tm2Eno/Tcf21tm2Eno (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS19: trunk mesenchyme Present Regionally restricted 3G There is asymmetric, left-sided expression in a region of undifferentiated mesoderm corresponding to the spleen anlage in the dorso-lateral wall of the stomach.
Specimen 3H: embryonic day 10.5; Tcf21tm2Eno/Tcf21tm2Eno (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS18: visceral pericardium Present Regionally restricted 3H Expression is present in the epicardium.
TS18: lung Present 3H
TS18: metanephric mesenchyme Present 3H
Specimen Not shown: embryonic day 13.5; Tcf21tm2Eno/Tcf21+ (more )
Structure Level Pattern Image Note
TS21: skeletal muscle Present Regionally restricted Expression is present in the jaw muscles.
TS21: tongue Present
TS21: visceral pericardium Present
TS21: esophagus mesenchyme Present Expression is present in the smooth muscle layers.
TS21: intestine mesenchyme Present Regionally restricted Expression is present in the smooth muscle layers of the gastrointestinal tract.
TS21: reproductive system Present Regionally restricted Expression is present in the smooth muscle layers of the urogenital tract.
Specimen 4A: embryonic day 13.5; Tcf21tm2Eno/Tcf21+ (more )
Structure Level Pattern Image Note
TS21: dorsal mesogastrium Present Regionally restricted 4A Expression is present in the spleen.
Specimen 4B: embryonic day 13.5; Tcf21tm2Eno/Tcf21tm2Eno (more )
Structure Level Pattern Image Note
TS21: dorsal mesogastrium Absent 4B There is only a faint trace of expression in an acellular region where the spleen should have formed.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory