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Gene Expression Data
Assay Details
Assay
Reference: J:99994 Ohinata Y, et al., Blimp1 is a critical determinant of the germ cell lineage in mice. Nature. 2005 Jul 14;436(7048):207-13
Assay type: RNA in situ
MGI Accession ID: MGI:3759960
Gene symbol: Prdm1
Gene name: PR domain containing 1, with ZNF domain
Probe: Blimp1 probe
Probe preparation: Antisense RNA
Results
Specimen 1a - Blimp1: embryonic day 7.25 (more )
Note: Late streak stage.
Structure Level Pattern Image Note
TS10: extraembryonic mesoderm Strong Regionally restricted 1a Blimp1 Expression is present 20-25 cells in the posterior-proximal extraembryonic mesoderm, where the founder primordial germ cells reside.
TS10: extraembryonic visceral endoderm Present 1a Blimp1
Specimen 1b - PS: embryonic day 6.0 (more )
Note: Prestreak stage.
Structure Level Pattern Image Note
TS8: extraembryonic visceral endoderm Present 1b PS
Specimen 1b - 0S: embryonic day 6.25 (more )
Note: No streak stage.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 1b OS Expression is present in the most proximal layer of the epiblast on the future posterior side where the primitive streak will form.
TS9: extraembryonic visceral endoderm Present 1b OS
Specimen 1b - ES: embryonic day 6.5 (more )
Note: Early streak stage.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 1b ES Expression is present in the most proximal layer of the epiblast on the future posterior side where the primitive streak will form.
TS9: extraembryonic visceral endoderm Present 1b ES
Specimen 1b - MS: embryonic day 6.75 (more )
Note: Midstreak stage.
Structure Level Pattern Image Note
TS10: extraembryonic mesoderm Present Regionally restricted 1b MS Expression is present in founder primordial germ cells.
TS10: extraembryonic visceral endoderm Present 1b MS
Specimen 1b - LS: embryonic day 7.25 (more )
Note: Late streak stage.
Structure Level Pattern Image Note
TS10: extraembryonic mesoderm Present Regionally restricted 1b LS Expression is present in founder primordial germ cells.
TS10: extraembryonic visceral endoderm Present 1b LS
Specimen 1b - 0B: embryonic day 7.25 (more )
Note: No bud stage.
Structure Level Pattern Image Note
TS10: extraembryonic mesoderm Present Regionally restricted 1b OB Expression is present in founder primordial germ cells.
TS10: extraembryonic visceral endoderm Present 1b OB
Specimen 1b - EB: embryonic day 7.5 (more )
Note: Early bud stage.
Structure Level Pattern Image Note
TS10: extraembryonic mesoderm Present Regionally restricted 1b EB Expression is present in founder primordial germ cells.
TS10: extraembryonic visceral endoderm Present 1b EB
Specimen 1c - left: embryonic day 6.25 (more )
Note: No streak stage. This is a proximal section.
Structure Level Pattern Image Note
TS9: embryo Absent 1c left
Specimen 1c - center: embryonic day 6.25 (more )
Note: No streak stage. This is an intermediate section.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 1c center Expression is present in the top layer of proximal epiblast cells on the future posterior side where the primitive streak will form.
TS9: extraembryonic visceral endoderm Present 1c center Expression in the visceral endoderm is on the opposite side of the embryo from the epiblast expression.
Specimen 1c - right: embryonic day 6.25 (more )
Note: No streak stage. This is a distal section.
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present 1c right
Specimen 1d - left: embryonic day 6.5 (more )
Note: Early streak stage. This is a proximal section.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 1d left Expression is present in the top layer of proximal epiblast cells on the future posterior side where the primitive streak will form.
TS9: embryo mesoderm Present Regionally restricted 1d left There is expression in a few cells in the nascent mesodermal region.
Specimen 1d - center: embryonic day 6.5 (more )
Note: Early streak stage. This is an intermediate section.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 1d center Expression is present in the top layer of proximal epiblast cells on the future posterior side where the primitive streak will form.
TS9: embryo mesoderm Present Regionally restricted 1d center There is expression in a few cells in the nascent mesodermal region.
TS9: extraembryonic visceral endoderm Present 1d center Expression in the visceral endoderm is on the opposite side of the embryo from the epiblast expression.
Specimen 1d - right: embryonic day 6.5 (more )
Note: Early streak stage. This is a distal section.
Structure Level Pattern Image Note
TS9: extraembryonic visceral endoderm Present 1d right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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