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Gene Expression Data
Assay Details
Assay
Reference: J:125491 McKnight KD, et al., Dynamic expression of Thyrotropin-releasing hormone in the mouse definitive endoderm. Dev Dyn. 2007 Oct;236(10):2909-17
Assay type: RNA in situ
MGI Accession ID: MGI:3759841
Gene symbol: Trh
Gene name: thyrotropin releasing hormone
Probe: Trh cDNA
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen Not shown: embryonic day 5.5 (more )
Structure Level Pattern Image Note
TS7: embryo Absent
Specimen 2A: embryonic day 6.25 (more )
Note: This embryo is at early streak stage. This is a lateral view.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 2A Expression is present in the epiblast.
TS9: extraembryonic ectoderm Absent 2A
TS9: extraembryonic visceral endoderm Absent 2A
TS9: primitive streak Absent 2A
TS9: extraembryonic component Absent 2A There is no expression in extraembryonic tissues.
Specimen 2B: embryonic day 6.25 (more )
Note: This embryo is at early streak stage. This is a transverse section.
Structure Level Pattern Image Note
TS9: embryo Present Regionally restricted 2B Expression is present in the epiblast.
TS9: extraembryonic ectoderm Absent 2B
TS9: extraembryonic visceral endoderm Absent 2B
TS9: primitive streak Absent 2B
Specimen 2C: embryonic day 6.75 (more )
Note: This embryo is at late streak stage. This is a lateral view.
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 2C Expression is present in the distal region of the epiblast.
TS10: definitive endoderm Present 2C There is expression on the lateral sides of the embryo. These cells are located in the outer endoderm layer and are believed to be the definitive endoderm cells.
Specimen 2D: embryonic day 6.75 (more )
Note: This embryo is at late streak stage. This is a transverse section.
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 2D Expression in the epiblast is now restricted to the distal region of the embryo.
Specimen 2E: embryonic day 6.75 (more )
Note: This embryo is at late streak stage. This is a lateral view.
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 2E Expression is present in the distal region of the epiblast.
TS10: definitive endoderm Present 2E There is expression on the lateral sides of the embryo. These cells are located in the outer endoderm layer and are believed to be the definitive endoderm cells.
Specimen 2F: embryonic day 6.75 (more )
Note: This embryo is at late streak stage. This is a transverse section.
Structure Level Pattern Image Note
TS10: definitive endoderm Present 2F There is expression on the lateral sides of the embryo. These cells are located in the outer endoderm layer and are believed to be the definitive endoderm cells.
Specimen 2G: embryonic day 6.75 (more )
Note: This embryo is at late streak stage. This is a transverse section.
Structure Level Pattern Image Note
TS10: embryo Present Regionally restricted 2G Expression is present in the distal region of the epiblast and on the lateral sides of the embryo.
TS10: primitive streak Absent 2G
Specimen 2H: embryonic day 7.5 (more )
Note: This embryo is at early bud stage. This is a lateral view.
Structure Level Pattern Image Note
TS11: definitive endoderm Strong Non-Uniform 2H Expression is present in the entire definitive endoderm; its level is higher in the anterior region of the embryo.
TS11: embryo Weak Regionally restricted 2H Expression is present at low levels in the remaining epiblast cells.
TS11: extraembryonic component Absent 2H
Specimen 2I: embryonic day 7.5 (more )
Note: This embryo is at early bud stage. This is a frontal view.
Structure Level Pattern Image Note
TS11: definitive endoderm Present 2I Expression is present in the entire definitive endoderm; its level is higher in the anterior region of the embryo.
TS11: extraembryonic visceral endoderm Absent 2I
TS11: notochordal plate Absent 2I
Specimen 2J: embryonic day 7.5 (more )
Note: This embryo is at early bud stage. This is a transverse section.
Structure Level Pattern Image Note
TS11: definitive endoderm Present 2J , 2K Expression is present in the entire definitive endoderm.
TS11: extraembryonic visceral endoderm Absent 2J , 2K
TS11: notochordal plate Absent 2J , 2K
Specimen 2L: embryonic day 7.5 (more )
Note: This embryo is at late bud stage. This is a lateral view.
Structure Level Pattern Image Note
TS11: definitive endoderm Strong Non-Uniform 2L Expression is enriched in the anterior definitive endoderm.
TS11: embryo Weak Regionally restricted 2L Expression is present at low levels in the remaining epiblast cells.
TS11: extraembryonic component Absent 2L
Specimen 2M: embryonic day 7.5 (more )
Note: This embryo is at late bud stage. This is a frontal view.
Structure Level Pattern Image Note
TS11: definitive endoderm Present Non-Uniform 2M Expression is enriched in the anterior definitive endoderm.
TS11: notochordal plate Absent 2M
Specimen 2N: embryonic day 7.5 (more )
Note: This embryo is at late bud stage. This is a transverse section.
Structure Level Pattern Image Note
TS11: definitive endoderm Present 2N
TS11: notochordal plate Absent 2N
Specimen 2O: embryonic day 7.5 (more )
Note: This embryo is at late bud stage. This is a transverse section.
Structure Level Pattern Image Note
TS11: definitive endoderm Present 2O
TS11: notochordal plate Absent 2O
Specimen 3A: embryonic day 7.75 (more )
Note: This embryo is at early head fold stage. This is a lateral view.
Structure Level Pattern Image Note
TS11: definitive endoderm Present Non-Uniform 3A Expression is present in the definitive endoderm with enrichment in the anterior near the head folds.
Specimen 3B: embryonic day 7.75 (more )
Note: This embryo is at early head fold stage. This is a frontal view.
Structure Level Pattern Image Note
TS11: definitive endoderm Present Non-Uniform 3B Expression is present in the definitive endoderm with enrichment in the anterior near the head folds.
Specimen 3C: embryonic day 7.75 (more )
Note: This embryo is at early head fold stage. This is a transverse section.
Structure Level Pattern Image Note
TS11: definitive endoderm Present 3C
TS11: notochordal plate Absent 3C There is no expression in the notochordal plate in the anterior midline.
Specimen 3D: embryonic day 7.75 (more )
Note: This embryo is at early head fold stage. This is a transverse section.
Structure Level Pattern Image Note
TS11: definitive endoderm Present 3D
TS11: notochordal plate Absent 3D There is no expression in the notochordal plate in the anterior midline.
Specimen 3E: embryonic day 7.75 (more )
Note: This embryo is at late head fold stage. This is a lateral view.
Structure Level Pattern Image Note
TS11: definitive endoderm Present 3E Expression is present in the entire definitive endoderm.
Specimen 3F: embryonic day 7.75 (more )
Note: This embryo is at late head fold stage. This is a frontal view.
Structure Level Pattern Image Note
TS11: definitive endoderm Present Regionally restricted 3F Expression is present in the definitive endoderm with enrichment in the anterior near the head folds.
Specimen 3G: embryonic day 7.75 (more )
Note: This embryo is at late head fold stage. This is a transverse section.
Structure Level Pattern Image Note
TS11: definitive endoderm Present 3H , 3G , 3I
TS11: node Absent 3G , 3I
TS11: notochordal plate Absent 3G , 3I
TS11: extraembryonic visceral endoderm Absent 3G , 3H
Specimen 3J: embryonic day 7.75 (more )
Note: This embryo is at late head fold stage. This is a transverse section.
Structure Level Pattern Image Note
TS11: definitive endoderm Present 3J , 3K
TS11: node Absent 3K , 3J
TS11: notochordal plate Absent 3K , 3J
Specimen 3L: embryonic day 7.75 (more )
Note: This embryo is at zero-somite stage. This is a lateral view.
Structure Level Pattern Image Note
TS11: definitive endoderm Present Non-Uniform 3L Expression is present in the entire definitive endoderm; it is enriched in the foregut invagination.
Specimen 3M: embryonic day 7.75 (more )
Note: This embryo is at zero-somite stage. This is a frontal view.
Structure Level Pattern Image Note
TS11: definitive endoderm Present Non-Uniform 3M Expression is enriched at the lip of the foregut invagination.
Specimen 3N: embryonic day 7.75 (more )
Note: This embryo is at zero-somite stage. This is a transverse section.
Structure Level Pattern Image Note
TS11: definitive endoderm Present Non-Uniform 3O , 3N Expression is present in the cells lining the foregut, with slightly higher expression in the ventral foregut.
TS11: notochordal plate Absent 3N , 3O Expression is absent from the cells at the dorsal midline of the foregut; they are believed to belong to the notochordal plate.
Specimen 3P: embryonic day 7.75 (more )
Note: This embryo is at zero-somite stage. This is a transverse section.
Structure Level Pattern Image Note
TS11: definitive endoderm Present Non-Uniform 3P Expression is present in the cells lining the foregut, with slightly higher expression in the ventral foregut.
TS11: notochordal plate Absent 3P Expression is absent from the cells at the dorsal midline of the foregut; they are believed to belong to the notochordal plate.
TS11: node Absent 3P
Specimen 4A: embryonic day 8.0 (more )
Note: This embryo is at four-somite stage. This is a lateral view.
Structure Level Pattern Image Note
TS12: gut endoderm Present 4A Expression is present in the entire definitive endoderm.
TS12: foregut diverticulum Strong 4A
TS12: hindgut diverticulum Strong 4A
Specimen 4B: embryonic day 8.0 (more )
Note: This embryo is at four-somite stage. This is a transverse section.
Structure Level Pattern Image Note
TS12: gut endoderm Present 4C , 4B Expression is present in the entire definitive endoerm.
TS12: foregut diverticulum Strong Non-Uniform 4B , 4C There is a higher level of expression in the ventral foregut.
TS12: notochordal plate Absent 4C , 4B Expression is absent from the cells at the dorsal midline of the foregut; they are believed to belong to the notochordal plate.
Specimen 4D: embryonic day 8.0 (more )
Note: This embryo is at four-somite stage. This is a transverse section.
Structure Level Pattern Image Note
TS12: gut endoderm Present 4D , 4E Expression is present in the entire definitive endoderm.
TS12: foregut diverticulum Strong 4E , 4D
TS12: notochordal plate Absent 4E , 4D Expression is absent from the cells at the dorsal midline of the foregut; they are believed to belong to the notochordal plate.
Specimen 4F: embryonic day 8.0 (more )
Note: This embryo is at four-somite stage. This is a transverse section.
Structure Level Pattern Image Note
TS12: gut endoderm Present 4F Expression is present in the entire definitive endoderm.
TS12: hindgut diverticulum Strong 4F
Specimen 4G: embryonic day 8.0 (more )
Note: This embryo is at six-somite stage. This is a lateral view.
Structure Level Pattern Image Note
TS12: foregut diverticulum Strong 4G
TS12: notochordal plate Absent 4G Expression is absent from the cells at the dorsal midline of the foregut; they are believed to belong to the notochordal plate.
TS12: hindgut diverticulum Strong 4G
TS12: neural tube Present 4G Expression is present in the posterior neural tube.
Specimen 4H: embryonic day 8.0 (more )
Note: This embryo is at six-somite stage. This is a transverse section.
Structure Level Pattern Image Note
TS12: foregut diverticulum Strong 4H There are higher levels of expression in the ventral foregut.
TS12: notochordal plate Absent 4H Expression is absent from the cells at the dorsal midline of the foregut; they are believed to belong to the notochordal plate.
Specimen 4I: embryonic day 8.0 (more )
Note: This embryo is at six-somite stage. This is a transverse section.
Structure Level Pattern Image Note
TS12: foregut diverticulum Strong 4I
TS12: notochordal plate Absent 4I Expression is absent from the cells at the dorsal midline of the foregut; they are believed to belong to the notochordal plate.
Specimen 4J: embryonic day 8.0 (more )
Note: This embryo is at six-somite stage. This is a transverse section.
Structure Level Pattern Image Note
TS12: neural tube Present 4J Expression is present in the posterior neural tube.
Specimen 4K: embryonic day 8.0 (more )
Note: This embryo is at six-somite stage. This is a transverse section.
Structure Level Pattern Image Note
TS12: hindgut diverticulum Strong 4K
Specimen 4L: embryonic day 9.0 (more )
Note: This is a lateral view.
Structure Level Pattern Image Note
TS14: future hindbrain Present 4L Expression is present at the midbrain-hindbrain junction.
TS14: future midbrain Present 4L Expression is present at the midbrain-hindbrain junction.
TS14: neural tube Present 4L Expression is present in the posterior neural tube.
TS14: gut Absent 4L Expression is no longer detected in the definitive endoderm.
Specimen 4M: embryonic day 9.0 (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS14: gut Absent 4M Expression is no longer detected in the definitive endoderm.
Specimen 4N: embryonic day 9.0 (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS14: gut Absent 4N Expression is no longer detected in the definitive endoderm.
Specimen 4O: embryonic day 9.0 (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS14: future hindbrain Present 4O Expression is present at the midbrain-hindbrain junction.
TS14: future midbrain Present 4O Expression is present at the midbrain-hindbrain junction.
Specimen 4P: embryonic day 9.0 (more )
Note: This is a transverse section.
Structure Level Pattern Image Note
TS14: neural tube Present 4P Expression is present in the posterior neural tube.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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01/06/2026
MGI 6.24 		The Jackson Laboratory