Antibody:	anti-ICAT
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: mesonephric tubule	Weak	Regionally restricted	1D	There is weak expression in epithelial cells.
TS17: nephric duct	Weak	Regionally restricted	1D	There is weak expression in epithelial cells of the Wolffian duct.
TS17: metanephric mesenchyme	Present		1D

Structure	Level	Pattern	Image	Note
TS19: ureteric bud	Strong	Regionally restricted	1E	There is intense expression in the epithelium in the tips.
TS19: nephric duct	Strong	Regionally restricted	1E	There is strong expression in the epithelium in the Wolffian duct.
TS19: metanephric mesenchyme	Strong		1E	There is strong expression in the condensed and dispersed mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: nephric duct	Present		1F	Expression is present in the epithelium in the Wolffian duct.
TS20: ureteric tip	Present		1F	Expression is high and stable in the ureteric bud epithelial cells.
TS20: renal vesicle	Present	Regionally restricted	1F	Expression is present in the epithelium of the renal vesicle.
TS20: mesenchyme of metanephros	Present		1F	Expression is present in the condensed and dispersed mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: ureteric tip	Present		1G	Expression is high and stable in the ureteric bud epithelial cells.
TS21: renal tubule	Weak	Regionally restricted	1G	There is weak expression in the epithelium.
TS21: renal vesicle	Weak	Regionally restricted	1G	There is weak expression in the epithelium.
TS21: mesenchyme of metanephros	Present		1G	There is expression in the condensed and dispersed mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: ureteric tip	Present	Regionally restricted	1H	Expression is high and stable in the ureteric bud epithelial cells.
TS22: cortical renal tubule	Absent		1H	There is no expression in the epithelium.
TS22: renal vesicle	Absent		1H	There is no expression in the epithelium.
TS22: mesenchyme of metanephros	Absent		1H	There is no expression in the condensed and dispersed mesenchyme.
TS22: maturing glomerular tuft	Present		1H