Gene Expression Data
Assay Details
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Assay
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Reference:
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J:76174
Chan EY, et al., Targeted disruption of Huntingtin-associated protein-1 (Hap1) results in postnatal death due to depressed feeding behavior. Hum Mol Genet. 2002 Apr 15;11(8):945-59
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3758033
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Gene symbol:
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Hap1
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Gene name:
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huntingtin-associated protein 1
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Antibody:
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CAG1-3
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Detection system:
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Secondary antibody coupled to rhodamine
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Results
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Specimen
8A:
embryonic day 17.5
(more  )
Specimen
8A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
not shown:
embryonic day 17.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
8B +/+:
embryonic day 17.5
(more )
Specimen
8B +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Non-Uniform |
8B +/+
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Expression levels were high in the dorsomedial nucleus and arcuate nucleus but were lower in the ventromedial hypothalamic nucleus.
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Specimen
8B -/-:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Hap1tm1Hay/Hap1tm1Hay
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
8C +/+ top:
embryonic day 17.5
(more )
Specimen
8C +/+ top:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
8C +/+ top
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Expression was detected in the arcuate nucleus. Subcellular localization of immunoreactivity was diffuse cytoplasmic staining in neurons. In addition, large cytoplasmic immunoreactive puncta were observed corresponding to stigmoid bodies.
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Specimen
8C +/- top:
embryonic day 17.5; Hap1tm1Hay/Hap1 +
(more )
Specimen
8C +/- top:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Hap1tm1Hay/Hap1+
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
8C +/- top
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Expression was detected in the arcuate nucleus as in +/+. Subcellular localization of immunoreactivity was diffuse cytoplasmic staining in neurons. Immunoreactive puncta were observed in fewer neurons in +/- embryos compared to +/+ embryos.
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Specimen
8C -/- top:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Hap1tm1Hay/Hap1tm1Hay
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
8C +/+ bottom:
embryonic day 17.5
(more )
Specimen
8C +/+ bottom:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
8C +/+ bottom
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Expression was detected in the dorsomedial nucleus. Subcellular localization of immunoreactivity was diffuse cytoplasmic staining in neurons. In addition, large cytoplasmic immunoreactive puncta were observed corresponding to stigmoid bodies.
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Specimen
8C +/- bottom:
embryonic day 17.5; Hap1tm1Hay/Hap1 +
(more )
Specimen
8C +/- bottom:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Hap1tm1Hay/Hap1+
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
8C +/- bottom
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Expression was detected in the dorsomedial nucleus as in +/+. Subcellular localization of immunoreactivity was diffuse cytoplasmic staining in neurons. Immunoreactive puncta were observed in fewer neurons in +/- embryos compared to +/+ embryos.
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Specimen
8C -/- bottom:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * ICR
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Mutant Allele(s):
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Hap1tm1Hay/Hap1tm1Hay
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
8D:
embryonic day 17.5
(more )
Specimen
8D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: hypothalamus |
Present |
Regionally restricted |
8D
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Subcellular localization of immunoreactivity in the hypothalamus was noted in a large stigmoid body in the perikarya of a neuron. Expression was confined to the cytoplasm.
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