Probe:	RATGAD 65/A
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Moderate	Regionally restricted	6a	Expression is present in a group of cells in the cerebellar differentiating zone.
TS20: midbrain	Present	Regionally restricted	6a	There is high-medium expression in tegmentum, belonging to perioculomotor nuclei, the mesencephalic reticular formation, the substantia nigra, and the ventral tegmental area. Weak expression is present in the superior colliculus.
TS20: forebrain	Present	Graded	6a	There is an inside-out expression gradient.
TS20: telencephalon	Present	Regionally restricted	6a	Expression is present in the ventral telencephalon.
TS20: lateral ganglionic eminence	Present	Regionally restricted	6a	Expression is present in the striatal differentiating zone.
TS20: hypothalamus	Present	Regionally restricted	6a	Expression is present in the anterior and posterior preoptic area and suprachiasmatic nuclei.
TS20: thalamus	Present	Regionally restricted	6a	Expression is present in the ventral thalamus. There is no expression in the principal thalamic nuclei.
TS20: medulla oblongata	Present	Regionally restricted	6a	Expression is present in the differentiating zone. There is no expression in domains in rhombomere 6.
TS20: dorsal root ganglion	Weak		6a
TS20: heart atrium	Present		6a
TS20: heart ventricle	Present		6a
TS20: cardiac muscle tissue	Present	Regionally restricted	6a	Expression is present in the trabecular myocardium.
TS20: blood vessel	Present	Regionally restricted	6a	Expression is present in major blood vessels.
TS20: midgut	Present		6a
TS20: midgut derived duodenum	Present		6a
TS20: stomach	Present		6a
TS20: liver	Present	Patchy	6a	Expression is present in isolated patches.
TS20: pancreas	Present	Patchy	6a	Expression is present in isolated patches.
TS20: medial ganglionic eminence	Present	Regionally restricted	6a	There is medium-high expression in the pallidal differentiating zone, with the strongest signal in the presumptive secondary proliferative population.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present		6b
TS20: midbrain	Present	Regionally restricted	6b	Medium-high expression is present in the pretectal area & dorsal tegmentum, belonging to perioculomotor nuclei, the mesencephalic reticular formation, the substantia nigra, & the ventral tegmental area. Weak expression is in the superior colliculus.
TS20: forebrain	Present	Graded	6b	There is an inside-out expression gradient.
TS20: medial ganglionic eminence	Moderate	Regionally restricted	6b	There is medium-high expression in the pallidal differentiating zone, with the strongest signal in the presumptive secondary proliferative population.
TS20: hypothalamus	Present	Regionally restricted	6b	Expression is seen in the anterior/posterior preoptic area, suprachiasmatic nuclei, & cell cord. Weak expression is in the retrochiasmatic & tuberal area. No expression is in the anterior dorsal nucleus, mammillary area or supraoptic-paraventricular area.
TS20: thalamus	Present	Regionally restricted	6b	Expression is present in the ventral thalamus, including in the subthalamic sulcus. There is no expression in the principal thalamic nuclei.
TS20: medulla oblongata	Present	Regionally restricted	6b	Expression is present in the differentiating zone.
TS20: spinal cord	Present	Regionally restricted	6b	Expression is present in the ventricular zone of the entire dorsal horn, in part of the intermediate zone, and in the corresponding differentiating zone. The ventral horn is nearly free of signal.
TS20: midgut	Present		6b
TS20: midgut derived duodenum	Present		6b
TS20: stomach	Present		6b
TS20: liver	Present	Patchy	6b	Expression is present in isolated patches.
TS20: pancreas	Present	Patchy	6b	Expression is present in isolated patches.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	7h	Expression is present in the ventricular zone of the entire dorsal horn, in part of the intermediate zone, and in the corresponding differentiating zone. The ventral horn is nearly free of signal.