Gene Expression Data
Assay Details
Assay
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Reference:
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J:123290
Hogarth CA, et al., Subcellular distribution of importins correlates with germ cell maturation. Dev Dyn. 2007 Aug;236(8):2311-20
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3722281
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Gene symbol:
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Kpna4
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Gene name:
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karyopherin subunit alpha 4
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Antibody:
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anti-importin alpha4
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
3C:
embryonic day 16.5
(more )
Specimen
3C:
(close )
Genetic Background:
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Swiss
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Age:
|
embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Female
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: germ cell of ovary |
Present |
Regionally restricted |
3C
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Expression is in the cytoplasm in germ cells.
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Specimen
3F:
embryonic day 16.5
(more )
Specimen
3F:
(close )
Genetic Background:
|
Swiss
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Age:
|
embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Male
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Type:
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section
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Fixation:
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Bouin's Fixative
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Embedding:
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Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: germ cell of testis |
|
Present |
Regionally restricted |
3F
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Expression is in the cytoplasm of germ cells.
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TS24: Leydig cell |
Leydig cell |
Present |
Regionally restricted |
3F
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Expression is in the cytoplasm of Leydig cells.
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TS24: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
3F
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Expression is in the cytoplasm Sertoli cells.
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Specimen
6E:
postnatal adult
(more )
Specimen
6E:
(close )
Genetic Background:
|
either: (C57BL/6 x CBA)F1 or Swiss
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Age:
|
postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
|
Bouin's Fixative
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Embedding:
|
Paraffin
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Specimen
6G:
postnatal adult
(more )
Specimen
6G:
(close )
Genetic Background:
|
either: (C57BL/6 x CBA)F1 or Swiss
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Age:
|
postnatal adult
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Sex:
|
Male
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Type:
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section
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Fixation:
|
Bouin's Fixative
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatid |
Strong |
Single cells |
6G
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Expression is in round spermatids.
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Specimen
6I:
postnatal adult
(more )
Specimen
6I:
(close )
Genetic Background:
|
either: (C57BL/6 x CBA)F1 or Swiss
|
Age:
|
postnatal adult
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Sex:
|
Male
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Type:
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section
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Fixation:
|
Bouin's Fixative
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Embedding:
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Paraffin
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Specimen
6K:
postnatal day 20
(more )
Specimen
6K:
(close )
Genetic Background:
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either: (C57BL/6 x CBA)F1 or Swiss
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Age:
|
postnatal day 20
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Sex:
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Male
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Type:
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section
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Fixation:
|
Bouin's Fixative
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Embedding:
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Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: spermatid |
|
Present |
|
6K
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Expression is in the nuclei of pachytene spermatocytes and round spermatids.
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TS28: spermatocyte |
|
Present |
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6K
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Expression is in the nuclei of pachytene spermatocytes and round spermatids.
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TS28: Sertoli cell |
Sertoli cell |
Present |
Single cells |
6K
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Expression is nuclear in Sertoli cells.
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Specimen
6M:
postnatal day 10
(more )
Specimen
6M:
(close )
Genetic Background:
|
either: (C57BL/6 x CBA)F1 or Swiss
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Age:
|
postnatal day 10
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Sex:
|
Male
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Type:
|
section
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Fixation:
|
Bouin's Fixative
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Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: Sertoli cell |
Sertoli cell |
Present |
Single cells |
6M
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Expression is cytoplasmic in Sertoli cells and is strong in the nuclei.
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