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Gene Expression Data
Assay Details
Assay
Reference: J:66592 Liu A, et al., EN and GBX2 play essential roles downstream of FGF8 in patterning the mouse mid/hindbrain region. Development. 2001 Jan;128(2):181-91
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:3720362
Gene symbol: En1
Gene name: engrailed 1
Reporter: lacZ
Direct Detection
Results
Specimen 2E: embryonic day 9.5; En1tm1Alj/En1+, En2tm1Alj/En2tm1Alj (more )
Structure Level Pattern Image Note
TS15: future hindbrain Present Regionally restricted 2E Expression was detected in a broad transverse band of cells across the mesencephalon/metencephalon junction.
TS15: future midbrain Present Regionally restricted 2E Expression was detected in a broad transverse band of cells across the mesencephalon/metencephalon junction.
Specimen 2E': embryonic day 9.5; En1tm2Alj/En1tm2Alj, En2tm2Alj/En2tm2Alj (more )
Structure Level Pattern Image Note
TS15: future hindbrain Present Regionally restricted 2E' Expression was strong in a small ventral midline patch around the mesencephalon/metencephalon junction and weak in a thin transverse band of cells at the anterior end of the hindbrain.
TS15: future midbrain Present Regionally restricted 2E' Expression was strong in a small ventral midline patch around the mesencephalon/metencephalon junction and weak in a thin transverse band of cells at the anterior end of the hindbrain.
Specimen 3A: embryonic day 8.0; En1tm1Alj/En1+, En2tm1Alj/En2tm1Alj (more )
Note: Embryo is at the 6 somite stage.
Structure Level Pattern Image Note
TS12: future hindbrain Present 3A
TS12: future midbrain Present 3A
Specimen 3A': embryonic day 8.0; En1tm2Alj/En1tm2Alj, En2tm2Alj/En2tm2Alj (more )
Note: Embryo is at the 6 somite stage.
Structure Level Pattern Image Note
TS12: future hindbrain Present 3A' Expression was detected in the mesencephalon/metencephalon, as in the heterozygous littermates.
TS12: future midbrain Present 3A' Expression was detected in the mesencephalon/metencephalon, as in the heterozygous littermates.
Specimen 3F: embryonic day 8.5; En1tm1Alj/En1+, En2tm1Alj/En2tm1Alj (more )
Note: Embryo is at the 11 somite stage.
Structure Level Pattern Image Note
TS13: future hindbrain Present 3F
TS13: future midbrain Present 3F
Specimen 3F': embryonic day 8.5; En1tm2Alj/En1tm2Alj, En2tm2Alj/En2tm2Alj (more )
Note: Embryo is at the 11 somite stage.
Structure Level Pattern Image Note
TS13: future hindbrain Present 3F' Expression was detected in the mesencephalon/metencephalon, as in the heterozygous littermates.
TS13: future midbrain Present 3F' Expression was detected in the mesencephalon/metencephalon, as in the heterozygous littermates.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory