Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:114758
Alcaraz WA, et al., Zfp423 controls proliferation and differentiation of neural precursors in cerebellar vermis formation. Proc Natl Acad Sci U S A. 2006;103(51):19424-19429
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3716190
|
|
Gene symbol:
|
Atoh1
|
|
Gene name:
|
atonal bHLH transcription factor 1
|
|
Probe:
|
Math1 probe5
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
Specimens were fixed in either 4% paraformaldehyde or 10% formalin, and embedded in 1:1 OCT:Aquamount.
|
|
|
Results
|
Specimen
8 Math1 E11.5 +/+ lateral:
embryonic day 11.5
(more  )
Specimen
8 Math1 E11.5 +/+ lateral:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E11.5 +/+ medial:
embryonic day 11.5
(more )
Specimen
8 Math1 E11.5 +/+ medial:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E11.5 nur12 lateral:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6 * C3H
|
|
Mutant Allele(s):
|
Zfp423nur12/Zfp423nur12
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E11.5 nur12 medial:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6 * C3H
|
|
Mutant Allele(s):
|
Zfp423nur12/Zfp423nur12
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E12.5 +/+ lateral:
embryonic day 12.5
(more )
Specimen
8 Math1 E12.5 +/+ lateral:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E12.5 +/+ medial:
embryonic day 12.5
(more )
Specimen
8 Math1 E12.5 +/+ medial:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E12.5 nur12 lateral:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6 * C3H
|
|
Mutant Allele(s):
|
Zfp423nur12/Zfp423nur12
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E12.5 nur12 medial:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6 * C3H
|
|
Mutant Allele(s):
|
Zfp423nur12/Zfp423nur12
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
Math1 E13.5 nur12 not shown:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6 * C3H
|
|
Mutant Allele(s):
|
Zfp423nur12/Zfp423nur12
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebellum |
Present |
Regionally restricted |
|
Authors report reduced expression levels beginning at E13.5.
|
Specimen
8 Math1 E14.5 +/+ lateral:
embryonic day 14.5
(more )
Specimen
8 Math1 E14.5 +/+ lateral:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E14.5 +/+ medial:
embryonic day 14.5
(more )
Specimen
8 Math1 E14.5 +/+ medial:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E14.5 nur12 lateral:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6 * C3H
|
|
Mutant Allele(s):
|
Zfp423nur12/Zfp423nur12
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E14.5 nur12 medial:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6 * C3H
|
|
Mutant Allele(s):
|
Zfp423nur12/Zfp423nur12
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E15.5 +/+ lateral:
embryonic day 15.5
(more )
Specimen
8 Math1 E15.5 +/+ lateral:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E15.5 +/+ medial:
embryonic day 15.5
(more )
Specimen
8 Math1 E15.5 +/+ medial:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
Specimen
8 Math1 E15.5 nur12 lateral:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6 * C3H
|
|
Mutant Allele(s):
|
Zfp423nur12/Zfp423nur12
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebellum |
Present |
Regionally restricted |
8_Math1 E15.5 nur12 lateral
|
Loss of expression more pronounced at E14.5 and E15.5, and more pronounced near the midline than laterally.
|
Specimen
8 Math1 E15.5 mur12 medial:
(close )
|
Genetic Background:
|
involves: BALB/c * C57BL/6 * C3H
|
|
Mutant Allele(s):
|
Zfp423nur12/Zfp423nur12
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Other - See Notes
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebellum |
Present |
Regionally restricted |
8_Math1 E15.5 nur12 medial
|
Loss of expression more pronounced at E14.5 and E15.5, and more pronounced near the midline than laterally.
|
|
Analysis Tools