Probe:

Lix1 cDNA1

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Present	Regionally restricted	1A' , 1A	Expression is in the ectodermal component.

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Regionally restricted	1B	Expression is in the ectodermal component.
TS13: future brain	Present	Regionally restricted	1B	Expression is in the headfold.
TS13: lateral plate mesoderm	Present		1B

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	1C	Expression is in the ectodermal component of the primitive streak.
TS15: future forebrain	Strong	Regionally restricted	1C	Expression is dorsal.
TS15: future midbrain	Strong	Regionally restricted	1C	Expression is dorsal.
TS15: future hindbrain	Strong	Regionally restricted	1C	Expression is in the dorsal metencephalon.
TS15: forelimb bud	Present		1C
TS15: hindlimb ridge	Present		1C

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present	Regionally restricted	1D	Expression is in the ectodermal component of the primitive streak.
TS13: lateral plate mesoderm	Present		1D

Structure	Level	Pattern	Image	Note
TS13: somatopleure	Present		1E
TS13: splanchnopleure	Present		1E

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted	1F	Expression is in the headfold.
TS13: lateral plate mesoderm	Present		1F

Structure	Level	Pattern	Image	Note
TS13: future brain	Present	Regionally restricted	1G	Expression is in the headfold.
TS13: lateral plate mesoderm	Present		1G

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Present
TS18: hindlimb bud	Present

Structure	Level	Pattern	Image	Note
TS20: brain	Present		2A E12.5

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		2A E13.5

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Present		2A E15.5
TS23: cerebral cortex subventricular zone	Present		2A E15.5
TS23: cortical plate	Present		2A E15.5

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present
TS20: genital tubercle	Present
TS20: lens vesicle	Present
TS20: spinal cord	Present

Structure	Level	Pattern	Image	Note
TS23: epithalamus mantle layer	Present
TS23: hypothalamus	Present
TS23: thalamus	Present	Regionally restricted		Expression is in the ventral thalamus.

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Present		2A P7
TS28: cortical layer I	Absent		2A P7
TS28: cortical layer II	Absent		2A P7
TS28: cortical layer III	Absent		2A P7
TS28: cortical layer IV	Absent		2A P7
TS28: cortical layer VI	Absent		2A P7

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Trace		2A P56

Structure	Level	Pattern	Image	Note
TS23: forebrain	Very strong	Regionally restricted	2B	Expression is in the hippocampus.
TS23: cerebral cortex intermediate zone	Present		2B
TS23: cerebral cortex subventricular zone	Present		2B
TS23: cortical plate	Present		2B
TS23: cerebral cortex	Present	Graded	2B	Expression is in a rostrocaudal gradient, with the younger, more caudal tissue (i.e., hippocampal region) showing much stronger expression than the developmentally more advanced rostral tissue.
TS23: corpus striatum	Present	Regionally restricted	2B	Expression is in the ventricular layer of the developing striatum.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Present		2C
TS28: subiculum	Present		2C
TS28: substantia nigra pars compacta	Present		2C
TS28: cortical layer V	Trace		2C

Structure	Level	Pattern	Image	Note
TS22: inferior colliculus	Present
TS22: midbrain tegmentum	Present
TS22: midbrain	Present	Regionally restricted		Expression is in the future substantia nigra.
TS22: hindbrain	Present	Regionally restricted		Expression is in a cell population rostrodorsal to the cerebellum and in the floor of the fourth ventricle along the midline.
TS22: cranial nerve	Strong	Regionally restricted		Expression is in differentiating cranial nerve nuclei.
TS22: metencephalon	Present	Regionally restricted		Expression is in the neuroepithelium corresponding to the rhombic lip, continuous with the mesencephalic domain.

Structure	Level	Pattern	Image	Note
TS23: midbrain	Present	Regionally restricted	3A	Expression is in the substantia nigra pars compacta.
TS23: midbrain tegmentum	Present	Regionally restricted	3A	Expression is in the ventral tegmental area.

Structure	Level	Pattern	Image	Note
TS23: hindbrain	Present	Regionally restricted	3B	Expression is in a cell population rostrodorsal to the cerebellum.
TS23: facial ganglion	Present	Regionally restricted	3B	Expression is in the motor nuclei.
TS23: trigeminal ganglion	Present	Regionally restricted	3B	Expression is in the motor nuclei.

Structure	Level	Pattern	Image	Note
TS23: glossopharyngeal ganglion	Present		3C
TS23: trigeminal ganglion	Present		3C

Structure	Level	Pattern	Image	Note
TS23: dorsal root ganglion	Strong		3D
TS23: dorsal grey horn	Present	Regionally restricted	3D	Expression is in dorsal horn interneurons.
TS23: basal columns	Present	Regionally restricted	3D	Expression is in a subset of motor neurons.

Structure	Level	Pattern	Image	Note
TS28: midbrain tegmentum	Present			Expression is in the ventral tegmental area.

Structure	Level	Pattern	Image	Note
TS28: substantia nigra pars compacta	Present
TS28: midbrain tegmentum	Present	Regionally restricted		Expression is in the ventral tegmental area.
TS28: hindbrain	Present	Regionally restricted		Expression is in a cell population rostrodorsal to the cerebellum.