Gene Expression Data
Assay Details
Assay
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Reference:
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J:45214
Dolle P, et al., Differential expression of genes encoding alpha, beta and gamma retinoic acid receptors and CRABP in the developing limbs of the mouse. Nature. 1989 Dec 7;342(6250):702-5
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3713206
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Gene symbol:
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Rarb
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Gene name:
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retinoic acid receptor, beta
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Probe:
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mRAR-beta
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
Not shown:
embryonic day 10.0
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 10.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
|
Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud |
Present |
Regionally restricted |
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Expression is detected only in the most proximal region of the limb buds and in the mesenchyme of the flank.
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Specimen
1e:
embryonic day 12.5
(more )
Note: This is a longitudinal section.
Specimen
1e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a longitudinal section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindlimb mesenchyme |
Present |
Regionally restricted |
1e RAR-beta
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Expression is present in a discrete, proximal region of the hindlimb mesenchyme, distinct but adjacent to the zeugopod precartilaginous blastema.
|
TS20: foot mesenchyme |
Present |
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1e RAR-beta
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Expression is present in several regions of loosely packed cells.
|
Specimen
Not shown:
embryonic day 12.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hand mesenchyme |
Present |
|
|
Expression is present in several regions of loosely packed cells.
|
Specimen
2a:
embryonic day 14.5
(more )
Note: This is a sagittal section.
Specimen
2a:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a sagittal section.
|
Specimen
2b:
embryonic day 14.5
(more )
Specimen
2b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2c:
embryonic day 14.5
(more )
Note: This is a transverse section.
Specimen
2c:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a transverse section.
|
|