Antibody:	anti-Frem1
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS17: eye surface ectoderm	Present	Regionally restricted	1B	Expression is in the basement membrane of epidermal epithelium.
TS17: optic cup	Present	Regionally restricted	1B	Expression is in retinal precursors.

Structure	Level	Pattern	Image	Note
TS20: cornea epithelium	Present	Regionally restricted	1F	Expression is in the basement membrane of the corneal ectoderm.
TS20: neural retinal epithelium	Present	Regionally restricted	1F	Expression is in the basement membrane of the inner retinal layer.

Structure	Level	Pattern	Image	Note
TS22: eyelid	Present	Regionally restricted	1J	Expression is in the basement membrane.
TS22: epidermis	Present	Regionally restricted	1J	Expression is in the basement membrane.
TS22: cornea	Present	Regionally restricted	1J	Expression is in the mesothelial layer of the cornea.
TS22: lens epithelium	Present	Regionally restricted	1J	Expression is in the cuboidal epithelium of lens.

Structure	Level	Pattern	Image	Note
TS24: eyelid	Present	Regionally restricted	1N	Expression is in the basement membrane.
TS24: conjunctival sac	Present	Regionally restricted	1N	Expression is in the conjuctive epithelium.
TS24: cornea epithelium	Present		1N

Structure	Level	Pattern	Image	Note
TS17: apical ectodermal ridge	Present	Regionally restricted	2B	Expression is in the basement membrane.

Structure	Level	Pattern	Image	Note
TS22: limb skin	Present	Regionally restricted	2F	Expression is in the basement membrane.

Structure	Level	Pattern	Image	Note
TS24: limb skin	Present	Regionally restricted	2J	Expression is in the basement membrane.

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present	Regionally restricted	3B	Expression is in the basement membrane.
TS20: visceral pleura	Present	Regionally restricted	3B	Expression is in the basement membrane.

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present	Regionally restricted	3F	Expression is in the basement membrane.
TS22: visceral pleura	Present	Regionally restricted	3F	Expression is in the basement membrane.

Structure	Level	Pattern	Image	Note
TS24: lung epithelium	Present	Regionally restricted	3J	Expression is in the basement membrane.
TS24: visceral pleura	Present	Regionally restricted	3J	Expression is in the basement membrane.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Regionally restricted	4B	Expression is in the basement membrane of the metanephric tubules.

Structure	Level	Pattern	Image	Note
TS23: metanephros	Present		4F

Structure	Level	Pattern	Image	Note
TS21: gut	Present		5B	Expression is in the epithelial basement membranes.

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Present		5F	Expression is in the epithelial basement membranes.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present		5J	Expression is in the epithelial basement membranes.