Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:117883
Chiotaki R, et al., Spatiotemporal distribution of Fras1/Frem proteins during mouse embryonic development. Gene Expr Patterns. 2007 Feb;7(4):381-8
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:3707471
|
Gene symbol:
|
Col7a1
|
Gene name:
|
collagen, type VII, alpha 1
|
Antibody:
|
anti-Col7a1
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor
|
Assay notes:
|
The authors state that expression was marginal between stages E10.5 and E16.5 (data not shown).
|
|
Results
|
Specimen
Not shown:
embryonic day 10.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
Not shown:
embryonic day 16.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: skin |
Trace |
|
|
|
Specimen
6A:
embryonic day 17.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: skin |
Strong |
Regionally restricted |
6A
|
Expression is in the basement membrane and connective tissue of dorsal skin.
|
Specimen
6B:
embryonic day 17.5
(more )
Specimen
6B:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 17.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: limb skin |
Strong |
Regionally restricted |
6B
|
Expression is in the basement membrane and connective tissue of dorsal skin.
|
Specimen
6C:
embryonic day 18.5
(more )
Specimen
6C:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|