Gene Expression Data
Assay Details
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Assay
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Reference:
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J:119269
Caqueret A, et al., Laminar organization of the early developing anterior hypothalamus. Dev Biol. 2006 Oct 1;298(1):95-106
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3704907
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Gene symbol:
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Nkx2-2
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Gene name:
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NK2 homeobox 2
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Results
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Specimen
4X:
embryonic day 11.5
(more  )
Specimen
4X:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
4X
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Expression was detected in a medial domain of the posterior aspect of the anterior hypothalamus.
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Specimen
4Z:
embryonic day 11.5
(more )
Note: Double labeled: red - Nkx2-2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
Specimen
4Z:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Nkx2-2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
4Z
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Expression was detected in a medial domain of the posterior aspect of the anterior hypothalamus.
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Specimen
5U:
embryonic day 12.5
(more )
Specimen
5U:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus mantle layer |
Present |
Regionally restricted |
5U
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Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
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Specimen
5W:
embryonic day 12.5
(more )
Note: Double labeled: red - Nkx2-2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
Specimen
5W:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Nkx2-2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hypothalamus mantle layer |
Present |
Regionally restricted |
5W
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Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
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Specimen
5AA:
embryonic day 13.5
(more )
Specimen
5AA:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus mantle layer |
Present |
Regionally restricted |
5AA
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Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
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Specimen
5AC:
embryonic day 13.5
(more )
Note: Double labeled: red - Nkx2-2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
Specimen
5AC:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 13.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Nkx2-2; green - Rgs4 (assay MGI:3703600). Expression domains were complementary.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: hypothalamus mantle layer |
Present |
Regionally restricted |
5AC
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Expression was not detected in the anterior aspect of the mantle layer of the anterior hypothalamus but was detected in the posterior aspect. Expression was restricted to the medial aspect of the neural tube.
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Specimen
7D:
embryonic day 15.5
(more )
Specimen
7D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Present |
Regionally restricted |
7D
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Expression was detected in a domain immediately adjacent to the third ventricle, which corresponds to the paraventricular nucleus.
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Specimen
7H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Sim1tm1Jlmd/Sim1tm1Jlmd
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Weak |
Regionally restricted |
7H
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Expression was reduced in the anterior hypothalamus of the mutant except in a subset of cells in a region corresponding to the lateral aspect of the paraventricular nucleus.
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Specimen
7N:
embryonic day 15.5
(more )
Specimen
7N:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Present |
Regionally restricted |
7N
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Expression was detected in a domain immediately adjacent to the third ventricle, which corresponds to the paraventricular nucleus.
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Specimen
7R:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Sim1tm1Jlmd/Sim1tm1Jlmd
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: hypothalamus |
Weak |
Regionally restricted |
7R
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Expression was reduced in the anterior hypothalamus of the mutant except in a subset of cells in a region corresponding to the lateral aspect of the paraventricular nucleus.
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