Probe:	Rspo3 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS11: allantois	Strong
TS11: primitive streak	Strong

Structure	Level	Pattern	Image	Note
TS13: allantois	Strong		1D
TS13: primitive streak	Strong		1D
TS13: somite	Strong		1D

Structure	Level	Pattern	Image	Note
TS13: allantois	Strong		1D'
TS13: primitive streak	Strong		1D'
TS13: somite	Strong		1D'

Structure	Level	Pattern	Image	Note
TS14: prosencephalon	Strong		1H
TS14: neural tube	Strong	Regionally restricted	1H	Expression is in the dorsal neural tube.
TS14: migrating neural crest	Present		1H
TS14: common atrial chamber	Present		1H
TS14: limb	Present		1H

Structure	Level	Pattern	Image	Note
TS17: forebrain	Strong		1L
TS17: neural tube	Strong	Regionally restricted	1L	Expression is in the dorsal neural tube.
TS17: common atrial chamber	Present		1L
TS17: limb	Present		1L

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Strong	Regionally restricted	2C	Expression is dorsal.

Structure	Level	Pattern	Image	Note
TS15: common atrial chamber	Present		2G

Structure	Level	Pattern	Image	Note
TS17: embryo ectoderm	Present		2K

Structure	Level	Pattern	Image	Note
TS14: primitive streak	Present	Regionally restricted		Expression is in both mesodermal and ectodermal cells in the caudal extremity.

Structure	Level	Pattern	Image	Note
TS15: tail unsegmented mesenchyme	Absent

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3C	Expression on the anterior side was more proximal, the posterior expression was extended to the distal end of limb buds.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud mesenchyme	Present	Regionally restricted	3G	Expression on the anterior side was more proximal, the posterior expression was extended to the distal end of limb buds.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit	Present	Regionally restricted	3K	Expression is in the interspaces between condensing mesenchymal cells of metacarpal/tarsals.
TS20: hindlimb digit	Present	Regionally restricted	3K	Expression is in the interspaces between condensing mesenchymal cells of metacarpal/tarsals.
TS20: forelimb interdigital region	Present		3K

Structure	Level	Pattern	Image	Note
TS22: forelimb phalanx cartilage condensation	Present	Regionally restricted	3O	Expression is in the ossification centers.
TS22: hand	Present	Regionally restricted	3O	Expression is in the joints, claw primordia, and perichondrium.

Structure	Level	Pattern	Image	Note
TS23: vibrissa	Present		4C

Structure	Level	Pattern	Image	Note
TS23: rib	Present	Regionally restricted	4G	Expression is in the perichondrium.

Structure	Level	Pattern	Image	Note
TS23: metanephros	Present	Regionally restricted	4K	Expression is in the peripheral nephric mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS23: pineal primordium	Strong	Regionally restricted	4O	Expression is in the ventral primordium.

Structure	Level	Pattern	Image	Note
TS23: forebrain	Strong	Regionally restricted	4S	Expression is in the hippocampus.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Strong
TS23: midbrain roof plate	Strong

Structure	Level	Pattern	Image	Note
TS25: humerus	Present	Regionally restricted	4W	Expression is in the prehypertrophic and hypertrophic chondrocytes of long bones.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Strong	Regionally restricted		Expression is in the hippocampus.
TS20: gut	Weak
TS20: heart	Weak
TS20: metanephros	Weak
TS20: tongue	Weak
TS20: vibrissa	Weak

Structure	Level	Pattern	Image	Note
TS23: forebrain	Strong	Regionally restricted		Expression is in the hippocampus.
TS23: cranium	Moderate
TS23: facial bone primordium	Moderate
TS23: gut	Moderate
TS23: metanephros	Moderate
TS23: pineal primordium	Moderate
TS23: vibrissa	Moderate
TS23: skeleton	Moderate	Regionally restricted		Expression is in the long bones.
TS23: heart	Weak
TS23: spinal cord	Weak
TS23: tongue	Weak
TS23: vertebra	Weak

Structure	Level	Pattern	Image	Note
TS25: forebrain	Moderate	Regionally restricted		Expression is in the hippocampus.
TS25: skeleton	Moderate	Regionally restricted		Expression is in the long bones.
TS25: cranium	Moderate
TS25: facial bone	Moderate
TS25: vertebra	Moderate
TS25: metanephros	Weak
TS25: pineal gland	Weak
TS25: spinal cord	Weak
TS25: vibrissa	Weak