Probe:	RIKEN clone G630034H08
Probe preparation:	Antisense RNA
Assay notes:	50-100 microns vibratome sections.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	1B	Expression is in the incipient mantle layer.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Absent
TS15: future midbrain	Absent

Structure	Level	Pattern	Image	Note
TS17: hindbrain mantle layer	Present	Regionally restricted	1C	Expression is absent in a region of the dorsoventral axis.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		1F

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present	Regionally restricted	1G	Expression is absent in the V2 interneuron column.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	1I	Expression is in the parabasal plate.

Structure	Level	Pattern	Image	Note
TS20: midbrain mantle layer	Strong	Regionally restricted	1K	Expression is at the interface between the ventricular and mantle layer.
TS20: midbrain ventricular layer	Strong	Regionally restricted	1K	Expression is at the interface between the ventricular and mantle layer.
TS20: thalamus mantle layer	Strong	Regionally restricted	1K	Expression is at the interface between the ventricular and mantle layer.
TS20: thalamus ventricular layer	Strong	Regionally restricted	1K	Expression is at the interface between the ventricular and mantle layer.

Structure	Level	Pattern	Image	Note
TS27: forebrain	Present	Regionally restricted	1O	Expression is in the subventricular zone, basal nucleaus of the stria terminalis, substantia innominate, prethalamus, and pretectum, and is absent in the basal plate.
TS27: amygdala	Present		1O
TS27: cerebral cortex	Present		1O
TS27: corpus striatum	Present		1O
TS27: hippocampus	Present		1O
TS27: main olfactory bulb	Present		1O
TS27: pallidum	Present		1O
TS27: thalamus	Present		1O
TS27: diencephalon	Present	Regionally restricted	1O	Expression is in the mammillary region and is absent in the tegmental area.
TS27: mammillary body	Present	Regionally restricted	1O	Expression is in the mammillary region and is absent in the tegmental area.
TS27: hypothalamus	Absent		1O

Structure	Level	Pattern	Image	Note
TS27: inferior colliculus	Present		1P
TS27: superior colliculus	Present		1P
TS27: substantia nigra	Present	Patchy	1P

Structure	Level	Pattern	Image	Note
TS27: hindbrain	Present	Regionally restricted	1Q	Expression is in the lemniscal nuclei, the sensory columns (cochlear, vestibular, trigeminal, and solitary tract), and is absent in the motor nuclei.
TS27: cerebellar cortex	Present		1Q
TS27: deep cerebellar nucleus	Present		1Q
TS27: gigantocellular reticular nucleus	Present		1Q
TS27: pontine nucleus	Present		1Q
TS27: superior olivary nucleus	Present		1Q

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Strong		1S
TS28: cerebral cortex	Strong		1S
TS28: cochlear nucleus	Strong		1S
TS28: hippocampus	Strong		1S
TS28: piriform cortex	Strong		1S
TS28: pontine nucleus	Strong		1S
TS28: superior olivary nucleus	Strong		1S
TS28: thalamus	Strong	Regionally restricted	1S	Expression is in some thalamic nuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebellar cortex	Present		2B

Structure	Level	Pattern	Image	Note
TS28: inferior colliculus	Present		2D

Structure	Level	Pattern	Image	Note
TS28: midbrain	Present	Regionally restricted	2F	Expression is in the mesencephalic sensitive nucleus of the trigeminus.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		2H

Structure	Level	Pattern	Image	Note
TS27: telencephalon	Strong	Regionally restricted	3A	Expression is in the dorsal subventricular zone.

Structure	Level	Pattern	Image	Note
TS27: telencephalon	Present	Regionally restricted	3B	Expression is in the subventricular zone.

Structure	Level	Pattern	Image	Note
TS27: telencephalon	Present	Regionally restricted	3C	Expression is in the subventricular zone.
TS27: cochlear nucleus	Present		3C

Structure	Level	Pattern	Image	Note
TS27: telencephalon	Present	Regionally restricted	3D	Expression is in the subventricular zone.
TS27: cochlear nucleus	Present		3D

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong	Regionally restricted	3E	Expression is in the inner part of the external granular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Strong		3F

Structure	Level	Pattern	Image	Note
TS28: diencephalon	Present	Regionally restricted	3G	In the diencephalic ependyma the cells contacting the ventriculum are not labeled while the cells in the immediate inner layer are labeled.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Single cells	4M

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Single cells	4N

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted	4O	Expression is in the vomeronasal nerve.

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb	Strong	Regionally restricted	4P	Expression is in the vomeronasal nerve.