Antibody:	Anti-ZO-1
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar dental lamina	Present	Regionally restricted	4A	Discontinuous signal was detected at the cell memebrane of dental lamina and dental papilla cells.
TS22: lower jaw molar dental papilla	Present	Regionally restricted	4A	Discontinuous signal was detected at the cell memebrane of dental lamina and dental papilla cells.
TS22: lower jaw molar epithelium	Present	Regionally restricted	4A	Expression in the inner and outer dental epithelium was continuously located at the epithelia-mesenchymal transition.
TS22: blood vessel	Present	Regionally restricted	4A	Expression was detected in the endothelial cells of the developing blood capillaries in dental papilla and dental follicle.

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar dental lamina	Present	Regionally restricted	4B	Discontinuous signal was detected at the cell memebrane of dental lamina and dental papilla cells.
TS22: lower jaw molar dental papilla	Present	Regionally restricted	4B	Discontinuous signal was detected at the cell memebrane of dental lamina and dental papilla cells.
TS22: lower jaw molar epithelium	Present	Regionally restricted	4B	Expression in the inner and outer dental epithelium was continuously located at the epithelia-mesenchymal transition.
TS22: blood vessel	Present	Regionally restricted	4B	Expression was detected in the endothelial cells of the developing blood capillaries in dental papilla and dental follicle.

Structure	Level	Pattern	Image	Note
TS25: stratum intermedium	Present	Regionally restricted	4C	Stratum intermedium cells had a punctuated signal.
TS25: lower jaw molar epithelium	Weak		4C
TS25: lower jaw molar mesenchyme	Present	Patchy	4C	Expression was detected in specific areas of the cell membrane.
TS25: blood vessel	Strong	Regionally restricted	4C	Expression was strong on the cell membrane of endothelial cells of blood capillaries of various types and caliber.

Structure	Level	Pattern	Image	Note
TS25: stratum intermedium	Present	Regionally restricted	4D	Stratum intermedium cells had a punctuated signal.
TS25: lower jaw molar epithelium	Weak		4D
TS25: lower jaw molar mesenchyme	Present	Patchy	4D	Expression was detected in specific areas of the cell membrane.
TS25: blood vessel	Strong	Regionally restricted	4D	Expression was strong on the cell membrane of endothelial cells of blood capillaries of various types and caliber.

Structure	Level	Pattern	Image	Note
TS26: lower jaw molar enamel organ	Present	Regionally restricted	4E	Expression was detected in preameloblast/ameloblast cells and signal accumulated at the basal and apical pole of the cell membrane. The intensity of the signal decreased at the tip of the dental cusps and in cervical loop direction.
TS26: stellate reticulum	Present	Regionally restricted	4E	Punctuated expression was detected in the stratum intermedium and stellate reticulum.
TS26: stratum intermedium	Present	Regionally restricted	4E	Punctuated expression was detected in the stratum intermedium and stellate reticulum.
TS26: blood vessel	Strong	Regionally restricted	4E	Expression was detected in endothelial cells of blood capillaries in the dental papilla subjacent to the odontoblasts and in microvessels inside the dental mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: lower jaw molar enamel organ	Present	Regionally restricted	4F	Expression was detected in preameloblast/ameloblast cells and signal accumulated at the basal and apical pole of the cell membrane. The intensity of the signal decreased at the tip of the dental cusps and in cervical loop direction.
TS26: stellate reticulum	Present	Regionally restricted	4F	Punctuated expression was detected in the stratum intermedium and stellate reticulum.
TS26: stratum intermedium	Present	Regionally restricted	4F	Punctuated expression was detected in the stratum intermedium and stellate reticulum.
TS26: blood vessel	Strong	Regionally restricted	4F	Expression was detected in endothelial cells of blood capillaries in the dental papilla subjacent to the odontoblasts and in microvessels inside the dental mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: lower jaw molar enamel organ	Present	Regionally restricted	4G	Expression was detected in preameloblast/ameloblast cells and signal accumulated at the basal and apical pole of the cell membrane. The intensity of the signal decreased at the tip of the dental cusps and in cervical loop direction.
TS26: stellate reticulum	Present	Regionally restricted	4G	Punctuated expression was detected in the stratum intermedium and stellate reticulum.
TS26: stratum intermedium	Present	Regionally restricted	4G	Punctuated expression was detected in the stratum intermedium and stellate reticulum.
TS26: blood vessel	Strong	Regionally restricted	4G	Expression was detected in endothelial cells of blood capillaries in the dental papilla subjacent to the odontoblasts and in microvessels inside the dental mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: lower jaw incisor epithelium	Present	Graded	8A	There was a gradient of signal detected in the inner dental epithelia cells of the labial side.
TS26: blood vessel	Strong	Regionally restricted	8A	Expression was strong in the cell membrane of endothelial capillaries in the dental mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: lower jaw incisor epithelium	Present	Graded	8B	There was a gradient of signal detected in the inner dental epithelia cells of the labial side.
TS26: blood vessel	Strong	Regionally restricted	8B	Expression was strong in the cell membrane of endothelial capillaries in the dental mesenchyme.