Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80666
Smits P, et al., The transcription factors L-Sox5 and Sox6 are essential for cartilage formation. Dev Cell. 2001 Aug;1(2):277-90
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3687185
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Gene symbol:
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Pcna
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Gene name:
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proliferating cell nuclear antigen
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Results
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Specimen
6C right panels:
embryonic day 12.5
(more  )
Note: Three panels are displayed; the top panel is a lower magnification image, the lower panels are higher magnification images.
Specimen
6C right panels:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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Note:
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Three panels are displayed; the top panel is a lower magnification image, the lower panels are higher magnification images.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: rib pre-cartilage condensation |
Present |
Regionally restricted |
6C right panels (3 panels)
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Expression was detected in chondroblasts in more advanced dorsal segments of the rib but was undetectable in prechondrocytes in the ventral segments.
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Specimen
6D middle panel:
embryonic day 16.5
(more )
Specimen
6D middle panel:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Strong |
Regionally restricted |
6D middle panel
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Expression was detected in periarticular reserve zone chondroblasts.
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Specimen
6D right panel:
embryonic day 16.5
(more )
Specimen
6D right panel:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Strong |
Regionally restricted |
6D right panel
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Expression was intense in columnar chondroblasts but was absent from hypertrophic chondrocytes.
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Specimen
6E right panel:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sox5tm1Vlf/Sox5tm1Vlf
Sox6tm1Vlf/Sox6tm1Vlf
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: limb digit |
Absent |
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6E right panel
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Expression was absent from digital rays.
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Specimen
6F right panel:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sox5tm1Vlf/Sox5tm1Vlf
Sox6tm1Vlf/Sox6tm1Vlf
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: Meckel's cartilage |
Present |
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6F right panel
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Expression was detected in most cells at levels close to those seen in wild type early differentiating chondroblasts.
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Specimen
6G right panel:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6
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Mutant Allele(s):
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Sox5tm1Vlf/Sox5tm1Vlf
Sox6tm1Vlf/Sox6tm1Vlf
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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10% Formalin
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: humerus |
Present |
Regionally restricted |
6G right panel
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Expression was detected in the lateral sides of epiphyseal cartilages but most cells in the core region were negative or weakly positive.
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Analysis Tools