Probe:	Cdca4 probe1
Probe preparation:	Antisense RNA
Assay notes:	CD-1 and C57BL/6 embryos were used in this assay. The probe was radio-labelled.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present		4A
TS17: 1st branchial arch maxillary component	Present		4A
TS17: branchial arch	Present		4A
TS17: fronto-nasal process	Present		4A
TS17: limb	Present		4A
TS17: brain	Present	Regionally restricted	4A	Expression was detected in the neuroepithelium.

Structure	Level	Pattern	Image	Note
TS19: fronto-nasal process	Present		4B
TS19: limb	Present		4B
TS19: liver	Present		4B
TS19: mandibular process	Present		4B
TS19: maxillary process	Present		4B
TS19: dorsal root ganglion	Weak		4B
TS19: midbrain ventricular layer	Present		4B	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS19: telencephalon ventricular layer	Present		4B	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS19: hindbrain	Present	Regionally restricted	4B	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS19: ear	Present	Regionally restricted	4B	Expression was detected in the otic vesicle.
TS19: eye	Present	Regionally restricted	4B	Expression was detected in the optic eminence.

Structure	Level	Pattern	Image	Note
TS21: head	Present	Regionally restricted	4C	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS21: head mesenchyme	Present	Regionally restricted	4C	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS21: nose	Present	Regionally restricted	4C	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS21: oral region	Present	Regionally restricted	4C	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS21: eye	Present	Regionally restricted	4C	Expression was detected in the optic eminence.
TS21: cerebral cortex ventricular layer	Present		4C	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS21: midbrain ventricular layer	Present		4C	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS21: hindbrain	Present	Regionally restricted	4C	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.

Structure	Level	Pattern	Image	Note
TS22: head	Present	Regionally restricted	4D	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS22: head mesenchyme	Present	Regionally restricted	4D	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS22: nose	Present	Regionally restricted	4D	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS22: oral region	Present	Regionally restricted	4D	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS22: incisor	Present		4D
TS22: molar	Present		4D
TS22: cerebral cortex ventricular layer	Present		4D	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS22: midbrain ventricular layer	Present		4D	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS22: hindbrain	Present	Regionally restricted	4D	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS22: nasal cavity	Present	Regionally restricted	4D	Expression was detected surrounding the nasal cavity.

Structure	Level	Pattern	Image	Note
TS22: head	Present	Regionally restricted	4E	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS22: head mesenchyme	Present	Regionally restricted	4E	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS22: nose	Present	Regionally restricted	4E	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS22: oral region	Present	Regionally restricted	4E	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS22: incisor	Present		4E
TS22: molar	Present		4E
TS22: cerebral cortex ventricular layer	Present		4E	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS22: midbrain ventricular layer	Present		4E	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS22: hindbrain	Present	Regionally restricted	4E	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.

Structure	Level	Pattern	Image	Note
TS23: head	Present	Regionally restricted	4F	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS23: head mesenchyme	Present	Regionally restricted	4F	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS23: nose	Present	Regionally restricted	4F	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS23: oral region	Present	Regionally restricted	4F	Expression was detected throughout the craniofacial mesenchyme and epithelia, although stronger towards the distal regions of the frontonasal prominences and mandibular and maxillary processes.
TS23: incisor	Present		4F
TS23: liver	Present		4F
TS23: molar	Present		4F
TS23: cerebral cortex ventricular layer	Present		4F	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS23: midbrain ventricular layer	Present		4F	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS23: hindbrain	Present	Regionally restricted	4F	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.

Structure	Level	Pattern	Image	Note
TS20: tongue	Present		4G
TS20: cerebral cortex ventricular layer	Present		4G	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS20: midbrain ventricular layer	Present		4G	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS20: hindbrain	Present	Regionally restricted	4G	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.

Structure	Level	Pattern	Image	Note
TS21: incisor	Present		4H
TS21: molar	Present		4H
TS21: tongue	Present		4H
TS21: cerebral cortex ventricular layer	Present		4H	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS21: midbrain ventricular layer	Present		4H	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS21: hindbrain	Present	Regionally restricted	4H	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS21: nasal cavity	Present	Regionally restricted	4H	Expression was detected surrounding the nasal cavity.

Structure	Level	Pattern	Image	Note
TS22: nasal cavity	Present	Regionally restricted	4I	Expression was detected surrounding the nasal cavity.
TS22: tongue	Present		4I
TS22: neural retinal epithelium	Present		4K , 4I
TS22: cerebral cortex ventricular layer	Present		4I	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS22: midbrain ventricular layer	Present		4I	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS22: hindbrain	Present	Regionally restricted	4I	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.

Structure	Level	Pattern	Image	Note
TS23: incisor	Present		4J , 4L
TS23: molar	Present		4J , 4L
TS23: nasal cavity	Present		4J	Expression was detected surrounding the nasal cavity.
TS23: neural retina	Present		4J
TS23: cerebral cortex ventricular layer	Present		4J	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS23: midbrain ventricular layer	Present		4J	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.
TS23: hindbrain	Present	Regionally restricted	4J	Expression was detected in the ventricular zone of the developing neocortex of the fore-, mid- and hindbrain.