Gene Expression Data
Assay Details
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Assay
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Reference:
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J:107704
Xiang M, Requirement for Brn-3b in early differentiation of postmitotic retinal ganglion cell precursors. Dev Biol. 1998 May 15;197(2):155-69
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3641273
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Gene symbol:
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Pou4f2
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Gene name:
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POU domain, class 4, transcription factor 2
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Antibody:
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anti-Brn-3b
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin
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Assay notes:
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Another antibody may have been used for this assay; see MGI:2448265.
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Results
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Specimen
1E:
embryonic day 11.5
(more  )
Specimen
1E:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: retina |
Present |
Regionally restricted |
1E Anti-Brn-3b
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Expression was detected in a small number of cells.
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Specimen
1F:
embryonic day 12.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: retina |
Present |
Regionally restricted |
1F Anti-Brn-3b
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Expression was detected in the central part of the retina in the ganglion cell layer and scattered cells of the proliferating ventricular zone.
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Specimen
1G:
embryonic day 13.5
(more )
Specimen
1G:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: retina |
Present |
Regionally restricted |
1G Anti-Brn-3b
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Expression was detected in the ganglion cell layer and in the ventricular zone.
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Specimen
1H:
embryonic day 15.5
(more )
Specimen
1H:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Regionally restricted |
1H Anti-Brn-3b
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Expression was detected in the ventricular zone.
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Specimen
1Q:
embryonic day 16.5
(more )
Specimen
1Q:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: retina |
Present |
Regionally restricted |
1Q Anti-Brn-3b
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Expression in the ventricular zone peaked at this stage.
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Specimen
1R:
embryonic day 19.5
(more )
Specimen
1R:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 19.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: retina |
Present |
Regionally restricted |
1R Anti-Brn-3b
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Expression was detected in occasional cells in the ventricular zone. There were severalfold more cells labeled in the late embryonic retina than in the early postnatal retina.
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Specimen
1S:
postnatal day 0
(more )
Specimen
1S:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: retina |
Present |
Regionally restricted |
1S Anti-Brn-3b
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Expression was detected in occasional cells in the ventricular zone.
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Specimen
1T:
postnatal day 5
(more )
Specimen
1T:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina |
Present |
Regionally restricted |
1T Anti-Brn-3b
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Expression was absent from the the outer layer of the retina. There were severalfold more cells labeled in the late embryonic retina than in the early postnatal retina.
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