Antibody:	anti-Brn-3a
Detection system:	Biotinylated secondary antibody/[Strept]avidin

Structure	Level	Pattern	Image	Note
TS19: retina	Absent		1A Anti-Brn-3a

Structure	Level	Pattern	Image	Note
TS20: retina	Absent		1B Anti-Brn-3a

Structure	Level	Pattern	Image	Note
TS21: retina	Present	Regionally restricted	1C Anti-Brn-3a	Expression was detected in differentiated ganglion cells in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS23: retina	Present	Regionally restricted	1D Anti-Brn-3a	Expression was detected in differentiated ganglion cells in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS24: retina	Present	Regionally restricted	1M Anti-Brn-3a	Expression was detected in differentiated ganglion cells in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS26: retina	Present	Regionally restricted	1N Anti-Brn-3a	Expression was detected in differentiated ganglion cells in the ganglion cell layer. Severalfold more cells were labeled in the late embryonic retina than in the early postnatal retina.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present	Regionally restricted	1O Anti-Brn-3a	Expression was detected in differentiated ganglion cells.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present	Regionally restricted	1P Anti-Brn-3a	Expression was detected in differentiated ganglion cells. Severalfold more cells were labeled in the late embryonic retina than in the early postnatal retina.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present	Regionally restricted	3I Anti-Brn-3a	Expression was detected in a large subset of cells.

Structure	Level	Pattern	Image	Note
TS27: retina ganglion cell layer	Present	Regionally restricted	3J Anti-Brn-3a	There were 4-6 fold fewer labeled cells in the mutant compared to control.

Structure	Level	Pattern	Image	Note
TS21: retina	Present	Regionally restricted	5A Anti-Brn3a	Expression was detected in ganglion cells.

Structure	Level	Pattern	Image	Note
TS21: retina	Present	Regionally restricted	5E Anti-Brn3a	There were 4-6 fold fewer labeled cells in the mutant retina compared to control.

Structure	Level	Pattern	Image	Note
TS23: retina	Present	Regionally restricted	5B Anti-Brn3a	Expression was detected in ganglion cells.

Structure	Level	Pattern	Image	Note
TS23: retina	Present	Regionally restricted	5F Anti-Brn3a	There were 4-6 fold fewer labeled cells in the mutant retina compared to control.

Structure	Level	Pattern	Image	Note
TS24: retina	Present	Regionally restricted	5C Anti-Brn3a	Expression was detected in ganglion cells.

Structure	Level	Pattern	Image	Note
TS24: retina	Present	Regionally restricted	5G Anti-Brn3a	There were 4-6 fold fewer labeled cells in the mutant retina compared to control.

Structure	Level	Pattern	Image	Note
TS26: retina	Present	Regionally restricted	5D Anti-Brn3a	Expression was detected in ganglion cells.

Structure	Level	Pattern	Image	Note
TS26: retina	Present	Regionally restricted	5H Anti-Brn3a	There were 4-6 fold fewer labeled cells in the mutant retina compared to control.