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Gene Expression Data
Assay Details
Assay
Reference: J:89199 Adams IR, et al., Identification and characterisation of mRif1: a mouse telomere-associated protein highly expressed in germ cells and embryo-derived pluripotent stem cells. Dev Dyn. 2004 Apr;229(4):733-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3618529
Gene symbol: Rif1
Gene name: replication timing regulatory factor 1
Antibody: Rif1 antibody
Detection system: Secondary antibody
Assay notes: Fixative was 3.7% formaldehyde in 0.1M potassium phosphate. Some ages were assigned by curator based on morphological criteria supplied by authors.
Results
Specimen 3A: postnatal (more )
Note: Germinal vesicle oocyte.
Structure Level Pattern Image Note
TS28: germ cell of ovary Present 3A Expression is in the nucleus of the cell but is excluded from the nucleolus.

Specimen 3B: postnatal (more )
Note: MII oocyte.
Structure Level Pattern Image Note
TS28: germ cell of ovary Present Uniform 3B

Specimen 3C: embryonic day 0.5 (more )
Note: 1-cell embryo (5-6hpf).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 3C Expression is in the pronuclei.

Specimen 3D: embryonic day 0.5 (more )
Note: 1-cell embryo (6-8hpf).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 3D Expression is in the pronuclei.

Specimen 3E: embryonic day 1.0 (more )
Note: 2-cell embryo.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 3E Expression is nuclear.
TS2: second polar body Absent 3E

Specimen 3F: embryonic day 1.0 (more )
Note: 4-cell embryo.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 3F Expression is nuclear.
TS3: second polar body Absent 3F

Specimen 3G: embryonic day 2.0 (more )
Note: 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present 3G Expression is nuclear.
TS3: second polar body Absent 3G

Specimen 3H: embryonic day 2.5 (more )
Note: Morula.
Structure Level Pattern Image Note
TS3: compacted morula Present 3H Expression is nuclear.
TS3: second polar body Absent 3H

Specimen 3I: embryonic day 3.5 (more )
Note: Blastocyst (3.5dpc).
Structure Level Pattern Image Note
TS5: inner cell mass Absent 3I
TS5: mural trophectoderm Present 3I Expression is in the nuclei.
TS5: polar trophectoderm Present 3I Expression is in the nuclei.

Specimen 3J: embryonic day 4.5 (more )
Note: Blastocyst (4.5 dpc).
Structure Level Pattern Image Note
TS6: trophectoderm Present 3J
TS6: epiblast Absent 3J
TS6: primitive endoderm Absent 3J

Specimen 4 5.5 dpc: embryonic day 5.5 (more )
Structure Level Pattern Image Note
TS8: epiblast Strong 4_5.5 dpc

Specimen 4 6.5 dpc: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: embryo ectoderm Present 4_6.5 dpc

Specimen 4 7.5 dpc: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Present 4_7.5 dpc
TS11: Reichert's membrane Weak 4_7.5 dpc
TS11: embryo mesoderm Absent 4_7.5 dpc
TS11: primordial germ cell Absent 4_7.5 dpc

Specimen 4 8.5 dpc: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: embryo Absent 4_8.5 dpc
TS13: primordial germ cell Absent 4_8.5 dpc

Specimen 4 9.5 dpc: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: hindgut epithelium Present Single cells 4_9.5 dpc Expression is in some cells in the hindgut epithelium.
TS15: primordial germ cell Present Single cells 4_9.5 dpc

Specimen 5 11.5 dpc Male: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 5_11.5 dpc male mRif1

Specimen 5 13.5 dpc Male: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: germ cell of testis Present 5_13.5 dpc male mRif1

Specimen 5 15.5 dpc Male: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: germ cell of testis Weak 5_15.5 dpc male mRif1

Specimen 5 17.5 dpc Male: embryonic day 17.5 (more )
Structure Cell Type Level Pattern Image Note
TS25: Sertoli cell Sertoli cell Weak 5_17.5 dpc male mRif1

Specimen 5 1 dpp Male: postnatal day 1 (more )
Structure Cell Type Level Pattern Image Note
TS27: Sertoli cell Sertoli cell Weak 5_1 dpp male mRif1 Low in Sertoli cells, absent in germ cells.
TS27: germ cell of testis Absent 5_1 dpp male mRif1 Low in Sertoli cells, absent in germ cells.

Specimen 5 5 dpp Male: postnatal day 5 (more )
Structure Cell Type Level Pattern Image Note
TS28: Sertoli cell Sertoli cell Present 5_5 dpp male mRif1
TS28: germ cell of testis Absent 5_5 dpp male mRif1

Specimen 5 10 dpp Male: postnatal day 10 (more )
Structure Cell Type Level Pattern Image Note
TS28: germ cell of testis Present 5_10 dpp male mRif1 Expression is in all the male germ cells toward the periphery of the testis cords at this stage but is absent from the more strongly GCNA-positive zygotene spermatocytes towards the center.
TS28: Sertoli cell Sertoli cell Present 5_10 dpp male mRif1

Specimen 5 11.5 dpc Female: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present 5_11.5 dpc female mRif1

Specimen 5 13.5 dpc Female: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: germ cell of ovary Present 5_13.5 dpc female mRif1

Specimen 5 15.5 dpc Female: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: germ cell of ovary Weak 5_15.5 dpc female mRif1

Specimen 5 17.5 dpc Female: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: germ cell of ovary Weak 5_17.5 dpc female mRif1
TS25: primary oocyte Weak 5_17.5 dpc female mRif1

Specimen 5 1 dpp Female: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: primary oocyte Weak 5_1 dpp female mRif1

Specimen 5 5 dpp Female: postnatal day 5 (more )
Structure Level Pattern Image Note
TS28: primary oocyte Present 5_5 dpp female mRif1

Specimen 5 10 dpp Female: postnatal day 10 (more )
Structure Level Pattern Image Note
TS28: primary oocyte Strong 5_10 dpp female mRif1 Expression is stronger in growing oocytes in primary follicles than in nongrowing oocytes in primordial follicles.

Specimen 6A: postnatal adult (more )
Structure Level Pattern Image Note
TS28: testis Present Regionally restricted 6A Expression is in cells located at the periphery of the testis cords.

Specimen 6B: postnatal adult (more )
Structure Level Pattern Image Note
TS28: testis Present Regionally restricted 6B Expression is in cells located at the periphery of the testis cords.

Specimen 6C: postnatal adult (more )
Structure Level Pattern Image Note
TS28: primary oocyte Present 6C Expression is in the nuclei of oocytes.
TS28: ovary follicle Weak 6C

Specimen 6D: postnatal adult (more )
Structure Level Pattern Image Note
TS28: oocyte of primordial follicle Present 6D Expression is in the nuclei of oocytes in primordial follicles.

Specimen 6E: postnatal adult (more )
Structure Level Pattern Image Note
TS28: primary oocyte Present 6E Expression is in the nuclei of growing oocytes in growing follicles.

Specimen 6F: postnatal adult (more )
Structure Level Pattern Image Note
TS28: primary oocyte Present 6F Expression is in the nuclei of growing oocytes in growing follicles.

Specimen 6G: postnatal adult (more )
Structure Level Pattern Image Note
TS28: oocyte of mature follicle Present 6G Expression is in the nuclei of fully grown oocytes in antral follicles.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory