Probe:	Tesr cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS19: gonad	Present		2A Male

Structure	Level	Pattern	Image	Note
TS19: gonad	Present		2A Female

Structure	Level	Pattern	Image	Note
TS20: testis	Present		2B Male

Structure	Level	Pattern	Image	Note
TS20: ovary	Weak		2B Female

Structure	Level	Pattern	Image	Note
TS20: testis	Present		2C Male

Structure	Level	Pattern	Image	Note
TS20: ovary	Trace		2C Female

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		2D Male

Structure	Level	Pattern	Image	Note
TS21: ovary	Trace		2D Female

Structure	Level	Pattern	Image	Note
TS21: primary sex cord	Present		3E

Structure	Cell Type	Level	Pattern	Image	Note
TS20: germ cell of testis		Present	Single cells	4A
TS20: Sertoli cell	Sertoli cell	Present	Single cells	4A

Structure	Level	Pattern	Image	Note
TS27: testis	Trace		6A	Faint to no expression.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: interstitium of the testis		Present	Single cells	6C	Expression is in the undifferentiated spermatogonia, Sertoli cells, and some interstitial cells.
TS27: spermatogonium		Present	Single cells	6C	Expression is in the undifferentiated spermatogonia, Sertoli cells, and some interstitial cells.
TS27: Sertoli cell	Sertoli cell	Present	Single cells	6C	Expression is in the undifferentiated spermatogonia, Sertoli cells, and some interstitial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: interstitium of the testis		Strong	Single cells	6E	Expression is in spermatogonia, Sertoli cells, and some interstitial cells.
TS28: spermatogonium		Strong	Single cells	6E	Expression is in spermatogonia, Sertoli cells, and some interstitial cells.
TS28: Sertoli cell	Sertoli cell	Strong	Single cells	6E	Expression is in spermatogonia, Sertoli cells, and some interstitial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: interstitium of the testis		Strong	Single cells	6G	Expression is from type A spermatogonia through the pachytene spermatocytes, Sertoli cells, and in some interstitial cells, absent in Leydig cells.
TS28: spermatocyte		Strong	Single cells	6G	Expression is from type A spermatogonia through the pachytene spermatocytes, Sertoli cells, and in some interstitial cells, absent in Leydig cells.
TS28: spermatogonium		Strong	Single cells	6G	Expression is from type A spermatogonia through the pachytene spermatocytes, Sertoli cells, and in some interstitial cells, absent in Leydig cells.
TS28: Sertoli cell	Sertoli cell	Strong	Single cells	6G	Expression is from type A spermatogonia through the pachytene spermatocytes, Sertoli cells, and in some interstitial cells, absent in Leydig cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: germ cell of testis		Present	Single cells	7A
TS28: Sertoli cell	Sertoli cell	Present	Single cells	7A
TS28: interstitium of the testis		Present	Regionally restricted	7A	Interstitial staining is seen in macrophages with no expression in Leydig cells.
TS28: Leydig cell	Leydig cell	Absent		7A	Interstitial staining is seen in macrophages with no expression in Leydig cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: germ cell of testis		Present	Single cells	7C
TS28: Sertoli cell	Sertoli cell	Present	Single cells	7C
TS28: interstitium of the testis		Present	Regionally restricted	7C	Interstitial staining is seen in macrophages with no expression in Leydig cells.
TS28: Leydig cell	Leydig cell	Absent		7C	Interstitial staining is seen in macrophages with no expression in Leydig cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: germ cell of testis		Present	Single cells	7E
TS28: Sertoli cell	Sertoli cell	Present	Single cells	7E
TS28: interstitium of the testis		Present	Regionally restricted	7E	Interstitial staining is seen in macrophages with no expression in Leydig cells.
TS28: Leydig cell	Leydig cell	Absent		7E	Interstitial staining is seen in macrophages with no expression in Leydig cells.

Structure	Level	Pattern	Image	Note
TS28: ovary	Present	Regionally restricted	8C , 8A	Expression is in granulosa, theca cells, and in corpus luteum.

Structure	Level	Pattern	Image	Note
TS19: eye	Present
TS19: head	Present

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted		Expression is in the neural arch, which arises from the dorsal side of the vertebra to enclose the spinal cord.
TS20: vertebral pre-cartilage condensation	Present
TS20: heart	Weak

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted		Expression is in the neural arch, which arises from the dorsal side of the vertebra to enclose the spinal cord.
TS21: vertebral pre-cartilage condensation	Present