Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:43761
Stoykova A, et al., Pax6-dependent regulation of adhesive patterning, R-cadherin expression and boundary formation in developing forebrain. Development. 1997 Oct;124(19):3765-77
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:3608682
|
|
Gene symbol:
|
Nes
|
|
Gene name:
|
nestin
|
|
Antibody:
|
clone RC2
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
Assay notes:
|
Antibody was applied after fixation in 2% paraformaldehyde.
|
|
|
Results
|
Specimen
10A:
embryonic day 16.5
(more )
Specimen
10A:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
10A
,
10B
|
Expression was detected in radial glia, whose fasciculated processes outline the cortico-striatal border.
|
Specimen
10C:
(close )
|
Genetic Background:
|
involves: C57BL/6J * DBA/2J
|
|
Mutant Allele(s):
|
Pax6Sey/Pax6Sey
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: telencephalon |
Present |
Regionally restricted |
10C
,
10D
|
Expression was detected in radial glial cells but fascicles could not be detected at the cortico-striatal border.
|
|