Gene Expression Data
Assay Details
Assay
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Reference:
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J:83132
Settle SH Jr, et al., Multiple joint and skeletal patterning defects caused by single and double mutations in the mouse Gdf6 and Gdf5 genes. Dev Biol. 2003 Feb 1;254(1):116-30
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3605540
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Gene symbol:
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Col2a1
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Gene name:
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collagen, type II, alpha 1
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Probe:
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pMCol2a1-1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1E:
embryonic day 14.5
(more )
Specimen
1E:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 14.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Structure |
Level |
Pattern |
Image |
Note |
TS22: hindlimb |
Present |
Regionally restricted |
1E
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Expression is in the skeletal elements.
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Specimen
3A WT:
embryonic day 13.5
(more )
Specimen
3A WT:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
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Embedding:
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Cryosection
|
Specimen
3B WT:
embryonic day 14.5
(more )
Specimen
3B WT:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Embedding:
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Cryosection
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Specimen
3E Mut:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Gdf6tm1Kng/Gdf6tm1Kng
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Age:
|
embryonic day 13.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
3F Mut:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
|
Gdf6tm1Kng/Gdf6tm1Kng
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Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
3I WT:
embryonic day 13.5
(more )
Specimen
3I WT:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
3J WT:
embryonic day 15.5
(more )
Specimen
3J WT:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
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Embedding:
|
Cryosection
|
Specimen
3M Mut:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
|
Gdf6tm1Kng/Gdf6tm1Kng
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
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Embedding:
|
Cryosection
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Specimen
3N Mut:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
|
Gdf6tm1Kng/Gdf6tm1Kng
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Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
section
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: talus |
Present |
Graded |
3N
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Expression was downregulated at the site of joints, but was otherwise continuously expressed in the central region.
|
TS23: tarsus |
Present |
Graded |
3N
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Expression was downregulated at the site of joints, but was otherwise continuously expressed in the central region.
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Specimen
7G:
embryonic day 16.5
(more )
Specimen
7G:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 16.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
section
|
Embedding:
|
Cryosection
|
|