Probe:	Klf15 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde. The author divided the genes analyzed in this publication into 4 groups based on their expression patterns. The expression pattern of this gene was placed in group 4 (See Table 1).

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	3A	Expression was detected in the neuroepithelium. The anterior expression limit coincided with the anterior border of the midbrain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	3B	Expression was detected in the neuroepithelium.
TS20: spinal cord floor plate	Present		3B	Expression was detected near midline in the floorplate of the neural tube.

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Present	Regionally restricted	3C	Expression was detected in the neuroepithelium of the entire central nervous system and more lateral from midline.
TS22: forebrain	Weak	Regionally restricted	3C	Expression was lighter in the ventricular zone of the neocortex and the anlage of the hippocampus.

Structure	Level	Pattern	Image	Note
TS22: liver	Present		3D
TS22: metanephros	Present		3D

Structure	Level	Pattern	Image	Note
TS22: nasal cavity epithelium	Present	Regionally restricted	3E	Expression was detected in the non-olfactory and olfactory epithelium of the nasal cavity.
TS22: olfactory epithelium	Present		3E

Structure	Level	Pattern	Image	Note
TS24: metanephros	Present		3F	Expression was increased from that seen at E14.5.
TS24: liver	Present		3F

Structure	Level	Pattern	Image	Note
TS20: liver	Weak

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Present	Regionally restricted		Expression was detected in the neuroepithelium of the entire central nervous system.
TS24: forebrain	Weak	Regionally restricted		Expression was lighter in the ventricular zone of the neocortex and the anlage of the hippocampus.
TS24: nasal cavity epithelium	Present	Regionally restricted		Expression was detected in the olfactory and non-olfactory epithelium.
TS24: olfactory epithelium	Present

Structure	Level	Pattern	Image	Note
TS26: central nervous system	Present	Regionally restricted		Expression was detected in the neuroepithelium of the entire central nervous system.
TS26: forebrain	Weak	Regionally restricted		Expression was lighter in the ventricular zone of the neocortex.
TS26: hippocampus	Weak
TS26: nasal cavity epithelium	Present	Regionally restricted		Expression was detected in the olfactory and non-olfactory epithelium of the nasal cavity.
TS26: liver	Present
TS26: olfactory epithelium	Present
TS26: metanephros	Present			Expression was increased from that seen at E14.5.