Gene Expression Data
Assay Details
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Assay
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Reference:
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J:81180
Avilion AA, et al., Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev. 2003 Jan 1;17(1):126-40
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3604678
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Gene symbol:
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Sox2
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Gene name:
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SRY (sex determining region Y)-box 2
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Antibody:
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FB46
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Detection system:
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Secondary antibody
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Results
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Specimen
5B:
embryonic day 3.5
(more  )
Specimen
5B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: inner cell mass |
Present |
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5B
,
5C
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The protein is present throughout the cells of the inner cell mass, primarily in the nucleus but also in the cytoplasm.
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| TS4: trophectoderm |
Present |
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5C
,
5B
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The protein is present in the cytoplasm, not the nucleus.
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Specimen
5L:
embryonic day 3.5
(more )
Specimen
5L:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: inner cell mass |
Present |
Uniform |
5L
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The protein is present throughout the cells of the inner cell mass, primarily in the nucleus but also in the cytoplasm.
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| TS4: trophectoderm |
Weak |
Regionally restricted |
5L
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There is weak expression in the extraembryonic endoderm/trophectoderm. It is believed it is of maternal origin as in situ hybridization fails to reveal the presence of any RNA.
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Specimen
5M:
embryonic day 0.5
(more )
Note: 1-cell embryo.
Specimen
5M:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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1-cell embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
Uniform |
5M
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The protein is present throughout the cell. It is believed to be of maternal origin.
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Specimen
5N:
embryonic day 1.0
(more )
Note: 2-cell embryo.
Specimen
5N:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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2-cell embryo.
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Specimen
5O:
embryonic day 2.0
(more )
Note: 6-cell embryo.
Specimen
5O:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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6-cell embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-8 cell stage embryo |
Present |
Non-Uniform |
5O
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The protein is present primarily in the nucleus, although some is present in the cytoplasm. It is believed to be of maternal origin.
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Specimen
5P:
embryonic day 2.5
(more )
Note: Early morula.
Specimen
5P:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Early morula.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
Non-Uniform |
5P
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The protein is present primarily in the nucleus, although some is present in the cytoplasm. It is believed to be of maternal origin.
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Specimen
5Q:
embryonic day 3.0
(more )
Note: Late morula.
Specimen
5Q:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Late morula.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: compacted morula |
Present |
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5Q
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The outer layer of cells show diffuse staining.
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Analysis Tools