Gene Expression Data
Assay Details
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Assay
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Reference:
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J:81180
Avilion AA, et al., Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev. 2003 Jan 1;17(1):126-40
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3604655
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Gene symbol:
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Sox2
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Gene name:
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SRY (sex determining region Y)-box 2
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Antibody:
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FB46
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
1G:
postnatal adult
(more  )
Specimen
1G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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1G
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Expression is present in the cytoplasm of oocytes, including those in developing primary follicles and early antral follicles.
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| TS28: ovary |
Present |
Regionally restricted |
1G
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Expression is present in some stromal cells.
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Specimen
1I:
postnatal adult
(more )
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Female
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: oocyte |
Present |
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1I
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Expression is present in the cytoplasm of oocytes, including those in mature antral follicles.
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| TS28: ovary |
Present |
Regionally restricted |
1I
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Expression is present in some stromal cells.
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Specimen
5W-1/2/3-wt:
embryonic day 3.5
(more )
Specimen
5W-1/2/3-wt:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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5W
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Three separate litters, progeny of heterozygous intercrosses, were analyzed. The protein was observed in all embryos, including presumably wild type embryos.
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Specimen
5W-1/2/3-het:
embryonic day 3.5; Sox2tm1Rlb/Sox2 +
(more )
Specimen
5W-1/2/3-het:
(close )
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Genetic Background:
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either: (involves: 129S/SvEv) or (involves: 129S/SvEv * MF1)
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Mutant Allele(s):
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Sox2tm1Rlb/Sox2+
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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5W
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Three separate litters, progeny of heterozygous intercrosses, were analyzed. The protein was observed in all embryos, including presumably heterozygotic embryos.
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Specimen
5W-1/2/3-hom:
(close )
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Genetic Background:
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either: (involves: 129S/SvEv) or (involves: 129S/SvEv * MF1)
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Mutant Allele(s):
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Sox2tm1Rlb/Sox2tm1Rlb
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Age:
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embryonic day 3.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: blastocyst |
Present |
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5W
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Three separate litters, progeny of heterozygous intercrosses, were analyzed. The protein was observed in all embryos, including presumably homozygotic embryos.
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Analysis Tools