Probe:	Sox2 cDNA9
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present	Non-Uniform		Expression is present in some cells.

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		1A
TS4: extraembryonic component	Absent		1A
TS4: trophectoderm	Absent		1A

Structure	Level	Pattern	Image	Note
TS9: embryo	Present		1B	Expression is present throughout the epiblast.
TS9: extraembryonic ectoderm	Strong	Regionally restricted	1B	Expression is present in a ring adjacent to the epiblast.

Structure	Level	Pattern	Image	Note
TS11: chorion ectoderm	Present		1C
TS11: neural ectoderm	Present	Regionally restricted	1C	Expression is present in the anterior, but not posterior, region of the presumptive neuroectoderm.
TS11: primitive streak	Absent		1C	There is no expression in cells ingressing through the primitive streak.

Structure	Level	Pattern	Image	Note
TS12: chorion ectoderm	Present		1D
TS12: neural ectoderm	Present	Regionally restricted	1D	Expression is present in the anterior region of the presumptive neuroectoderm.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Present	Regionally restricted	1E	Expression is present in the extraembryonic ectoderm portion of the chorion.
TS13: neural tube	Present		1E
TS13: head	Present		1E	Expression is present in the headfolds.

Structure	Level	Pattern	Image	Note
TS15: future brain	Present		1F	Expression is present throughout the nervous system.
TS15: neural tube	Present		1F	Expression is present throughout the nervous system.
TS15: sensory organ system	Present		1F	Expression is present in the sensory placodes
TS15: branchial arch	Present		1F
TS15: gut	Present		1F	Expression is present in gut endoderm.

Structure	Level	Pattern	Image	Note
TS15: primordial germ cell	Present			Expression is present in male and female germ cells.

Structure	Level	Pattern	Image	Note
TS11: chorion	Present		6A left
TS11: embryo	Present		6A left	Expression is present throughout the epiblast.