Probe:	eHand cDNA6
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS11: trophectoderm	Strong		2A/B	Expression was at high levels in all trophoblast cells.
TS11: ectoplacental cone	Strong	Regionally restricted	2A/B	Expression is restricted to trophoblast cells.
TS11: chorion	Present		2A/B
TS11: extraembryonic mesoderm	Present		2A/B
TS11: yolk sac	Present		2A/B
TS11: embryo	Absent		2A/B

Structure	Level	Pattern	Image	Note
TS13: heart	Strong		2C/D
TS13: pericardial component	Strong		2C/D
TS13: lateral plate mesoderm	Strong	Regionally restricted	2C/D	Expression is present in distal region.
TS13: extraembryonic component	Present		2C/D	Expression is present in trophoblast cells and extraembryonic membranes, including the yolk sac, throughout development.
TS13: neural tube	Absent		2C/D

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Present		2E/F
TS15: extraembryonic component	Present		2E/F	Expression is present in trophoblast cells and extraembryonic membranes, including the yolk sac, throughout development.
TS15: central nervous system	Absent		2E/F

Structure	Level	Pattern	Image	Note
TS17: sympathetic nervous system	Present		3A/B	Expression is present in the sympathetic ganglion primordium.
TS17: limb	Weak	Regionally restricted	3A/B	Expression is present in the distal posterior region of the limb bud.
TS17: central nervous system	Absent		3A/B
TS17: dorsal root ganglion	Absent		3A/B	Expression is not seen in sensory or motor ganglia (STRUCTURE?)

Structure	Level	Pattern	Image	Note
TS17: heart	Present

Structure	Level	Pattern	Image	Note
TS19: autonomic nervous system	Strong	Regionally restricted	3C/D	Expression is present in both autonomic ganglion and enteric system.
TS19: enteric nervous system	Strong	Regionally restricted	3C/D	Expression is present in both autonomic ganglion and enteric system.
TS19: thoracic ganglion	Strong		3C/D	Expression is present in the sympathetic trunk.
TS19: 1st branchial arch	Strong		3C/D	Expression is present in the first pharyngeal arch.
TS19: outflow tract	Present		3C/D
TS19: hindgut	Strong		3C/D
TS19: midgut	Strong		3C/D

Structure	Level	Pattern	Image	Note
TS20: tongue	Present	Regionally restricted	3E/F	Expression is present in septal regions of the tongue.
TS20: mandibular process	Present		3E/F
TS20: nasal cavity epithelium	Absent		3E/F	Expression is present in the neural epithelium of the developing nose.
TS20: head	Absent		3E/F	There is no expression in the developing facial skeleton.
TS20: skeleton	Absent		3E/F	There is no expression in the developing facial skeleton.
TS20: central nervous system	Absent		3E/F

Structure	Level	Pattern	Image	Note
TS21: heart valve	Present		4A/B
TS21: intestine	Present		4A/B
TS21: mandible	Present		4A/B
TS21: tongue	Present		4A/B
TS21: thoracic ganglion	Present		4A/B	Expression is present in the sympathetic trunk. Ganglia showing expression include the stellate and paraaortic.
TS21: enteric nervous system	Present	Regionally restricted	4A/B	Expression is present in the enteric system.
TS21: gland	Present	Regionally restricted	4A/B	Expression is present in the adrenal medulla. STRUCTURE

Structure	Level	Pattern	Image	Note
TS22: heart valve	Present		4C/D
TS22: mandible	Present		4C/D
TS22: tongue	Present		4C/D
TS22: thoracic ganglion	Present		4C/D	Expression is present in the sympathetic trunk.
TS22: enteric nervous system	Present		4C/D	Expression is present in the enteric system.
TS22: cranial nerve	Absent		4C/D

Structure	Level	Pattern	Image	Note
TS23: thoracic ganglion	Present		5A/D	Expression is present in the sympathetic trunk. Ganglia expressing include the superior cervical.
TS23: central nervous system	Absent		5A/D

Structure	Level	Pattern	Image	Note
TS23: adrenal medulla	Strong	Single cells	5B/E	Expression is present in clusters of cells.

Structure	Level	Pattern	Image	Note
TS23: enteric nervous system	Present		5C/F	Expression is present in peripheral layers of tissue containing smooth muscle and the enteric neural plexus.
TS23: smooth muscle tissue	Present		5C/F	Expression is present in peripheral layers of tissue containing smooth muscle and the enteric neural plexus.

Structure	Level	Pattern	Image	Note
TS28: adrenal gland	Absent
TS28: intestine	Absent