Antibody:	anti-5T4
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: uterus	Absent		4A(ii) , 4A(i)	Absent in the non-pregnant uterus.

Structure	Level	Pattern	Image	Note
TS28: endometrium luminal epithelium	Strong	Regionally restricted	4B(i) , 4B(ii)	Expression is in the uterine luminal epithelium in this E4.5 pregnant mouse.

Structure	Level	Pattern	Image	Note
TS6: trophectoderm	Present	Single cells

Structure	Level	Pattern	Image	Note
TS8: extraembryonic endoderm	Present		4C
TS8: polar trophectoderm	Present		4C

Structure	Level	Pattern	Image	Note
TS8: polar trophectoderm	Present

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Present	Regionally restricted		Expression is present in the ectoplacental cone that is derived from polar trophectoderm.

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Regionally restricted	5A	Expression is in the lateral and ventral areas.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Present	Regionally restricted	5B	Expression is in the basal layer.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Regionally restricted	5C	Expression is in the basal layer.

Structure	Level	Pattern	Image	Note
TS21: mesothelium	Present		5D

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present		5E

Structure	Level	Pattern	Image	Note
TS21: oral epithelium	Present		5F

Structure	Level	Pattern	Image	Note
TS22: tongue epithelium	Present		5G

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Present		5H	Expression is in both the inner and outer root sheats.

Structure	Level	Pattern	Image	Note
TS23: stomach epithelium	Present		5I

Structure	Level	Pattern	Image	Note
TS23: maturing glomerular tuft	Present	Regionally restricted	5J	Expression is in some glomeruli.

Structure	Level	Pattern	Image	Note
TS24: intestine epithelium	Present		5K

Structure	Level	Pattern	Image	Note
TS24: palate epithelium	Present		5L
TS24: tongue epithelium	Present		5L

Structure	Level	Pattern	Image	Note
TS20: epithelium	Present	Regionally restricted		Authors state that high levels of expression were increasingly confined to the basal layer of all stratified epithelia and also detected in most nonstratified epithelia.
TS20: metanephros	Weak	Regionally restricted		Expression in condensing mesenchyme of the kidney primordium.

Structure	Level	Pattern	Image	Note
TS21: skin	Present	Regionally restricted		Expression is strong and restricted to the basal layer ventrally, while dorsally, expression was relatively weak and punctate.
TS21: epithelium	Present	Regionally restricted		Authors state that high levels of expression were increasingly confined to the basal layer of all stratified epithelia and also detected in most nonstratified epithelia.
TS21: vibrissa follicle	Present			Expression is in both the inner and outer root sheats.

Structure	Level	Pattern	Image	Note
TS22: epithelium	Present	Regionally restricted		Authors state that high levels of expression were increasingly confined to the basal layer of all stratified epithelia and also detected in most nonstratified epithelia.
TS22: lung	Present	Regionally restricted		Expression is in the bronchi, but not all bronchioles.
TS22: maturing glomerular tuft	Present	Regionally restricted		Expression is in some glomeruli.
TS22: epidermis	Strong	Regionally restricted		Expression is in the basal layer.

Structure	Level	Pattern	Image	Note
TS23: vibrissa follicle	Present	Regionally restricted		Expression is in the outer root sheath.
TS23: epithelium	Present	Regionally restricted		Authors state that high levels of expression were increasingly confined to the basal layer of all stratified epithelia and also detected in most nonstratified epithelia.
TS23: lung	Present	Regionally restricted		Expression is in the bronchi, but not all bronchioles.
TS23: pancreas	Present	Regionally restricted		Expression is in epithelium.
TS23: epidermis	Strong	Regionally restricted		Expression is in the basal layer.

Structure	Level	Pattern	Image	Note
TS24: epithelium	Present	Regionally restricted		Authors state that high levels of expression were increasingly confined to the basal layer of all stratified epithelia and also detected in most nonstratified epithelia.
TS24: vibrissa follicle	Present	Regionally restricted		Expression is in the outer root sheath.
TS24: lung	Present	Regionally restricted		Expression is in the bronchi, but not all bronchioles.
TS24: pancreas	Present	Regionally restricted		Expression is in epithelium.
TS24: maturing glomerular tuft	Present	Regionally restricted		Expression is in some glomeruli.
TS24: epidermis	Strong	Regionally restricted		Expression is in the basal layer.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Present	Regionally restricted	7B , 7A	Expression is in a stripe near the lateral ventricle.
TS22: lateral ventricle	Strong		7B , 7A

Structure	Level	Pattern	Image	Note
TS22: midbrain roof plate	Present		7G

Structure	Level	Pattern	Image	Note
TS22: 3rd ventricle	Present	Regionally restricted	7H	Expression is in the cuboidal epithelial ependymal layer.
TS22: lateral ventricle	Present	Regionally restricted	7H	Expression is in the cuboidal epithelial ependymal layer.
TS22: choroid plexus	Present		7H

Structure	Level	Pattern	Image	Note
TS27: brain	Strong	Regionally restricted	8A , 8B	Expression is around lateral ventricles.
TS27: choroid plexus	Present		8A , 8B

Structure	Level	Pattern	Image	Note
TS27: brain ependyma	Present
TS27: brain	Present	Regionally restricted		Expression is in the roof plate.

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Present